We constructed a preparative instrumentation and developed the methods that are based on separation of the samples by bidirectional isotachophoresis/moving boundary electrophoresis in continuous divergent flow. The described instrumentation can be used for a variety of the samples, however, it can be easily optimized and tailored for the specific sample. The trapezoid separation bed from nonwoven textile exhibited minimum adsorption effect for sample and it can be used repeatedly. By the addition of different spacers via separation space inlets, the sections of pH gradient can be modified to enhance the separation. The liquid flow from two inlets positioned on each side of the sample inlet prevented the contact of the sample with anolyte and catholyte at the analysis beginning. One pair of thin electrodes (graphite and stainless-steel) was placed at the separation space output. The electrode products were washed out into drains without disturbing the focusing process. The influence of EOF was managed by tilting the separation bed in the direction from cathodic to anodic side. The components of spirulina supernatant and color pI markers were separated in the pH gradient from 3.9 to 10.1. pH gradient was stable for at least 4.5 h and spirulina supernatant from about 0.12 g of dry powder was processed. Compared to other preparative methods used for spirulina separation, the presented method/instrumentation working with a continuous divergent flow had essential advantages. The efficient separation was fast, and no intermediate steps were necessary to obtain liquid fractions with separated components compatible with further biological experiments.

中文翻译：

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用于纯化生物样品的制备型连续流动电泳仪器

我们构建了一个制备仪器并开发了基于在连续发散流中通过双向等速电泳/移动边界电泳分离样品的方法。所描述的仪器可用于各种样品，但是，它可以很容易地针对特定样品进行优化和定制。无纺布梯形分离床对样品的吸附作用最小，可重复使用。通过通过分离空间入口添加不同的垫片，可以修改 pH 梯度部分以增强分离。来自位于样品入口两侧的两个入口的液流防止样品在分析开始时与阳极液和阴极液接触。一对薄电极（石墨和不锈钢）放置在分离空间的输出端。电极产品在不干扰聚焦过程的情况下被冲入排水管。EOF 的影响是通过在从阴极侧到阳极侧的方向倾斜分离床来控制的。在 3.9 至 10.1 的 pH 梯度中分离螺旋藻上清液和颜色 pI 标记的成分。pH 梯度稳定至少 4.5 小时，处理来自约 0.12 g 干粉的螺旋藻上清液。与用于螺旋藻分离的其他制备方法相比，所提出的使用连续发散流的方法/仪器具有重要的优势。高效分离速度快，