Through the process of chemotactic migration, neutrophils are able to recruit to an inflammatory site and eliminate pathogens, thus playing a vital role in host defense. The process of neutrophil chemotaxis is mediated by dynamic actin reorganization and polymerization. Adseverin, an actin-binding protein and member of the Gelsolin superfamily of proteins, has been hypothesized to regulate goal directed movement through the capping and severing of actin filaments, but has never been investigated in the context of neutrophil chemotaxis. Using an Adseverin knockout mouse model, we show that Adseverin plays a role in subcortical F-actin assembly at the leading edge during chemotaxis through the generation of free barbed ends on existing actin filaments. In addition, in the absence of Adseverin, neutrophils show reduced speed of migration in vitro and in vivo. This study indicates that Adseverin is a regulator of actin filament generation during neutrophil chemotaxis.

中文翻译：

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肌动蛋白结合蛋白 Adseverin 介导中性粒细胞极化和迁移

通过趋化迁移过程，中性粒细胞能够募集到炎症部位并消除病原体，从而在宿主防御中发挥重要作用。中性粒细胞趋化过程由动态肌动蛋白重组和聚合介导。Adseverin 是一种肌动蛋白结合蛋白和凝溶胶蛋白超家族的成员，已被假设通过加帽和切断肌动蛋白丝来调节目标定向运动，但从未在中性粒细胞趋化性的背景下进行过研究。使用 Adseverin 敲除小鼠模型，我们表明 Adseverin 通过在现有肌动蛋白丝上产生游离倒刺末端在趋化过程中在前沿的皮层下 F-肌动蛋白组装中发挥作用。此外，在没有 Adseverin 的情况下，中性粒细胞在体外和体内显示出迁移速度降低。该研究表明 Adseverin 是中性粒细胞趋化过程中肌动蛋白丝生成的调节剂。