Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point-of-care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three-dimensional hydrodynamic focusing system and an on-chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath flow and sample flow, the blood cells can be horizontally and vertically focused in the center of microchannel. Optical fibers and on-chip microlens are embedded for the excitation and detection of single-cell. The microfluidic chip was validated by classifying white blood cells from clinical blood samples.

中文翻译：

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用于白细胞分析的微流控细胞仪

尽管细胞计数仪广泛用于白细胞分类，但传统细胞仪在床旁检测中的性能仍有待提高。微流体技术已在便携式设备的开发中显示出巨大的潜力。在这里，我们展示了一个微流控细胞仪的原型，它集成了一个三维流体动力学聚焦系统和一个片上光学系统来对白细胞进行计数和分类。通过调节鞘流和样品流的流速，血细胞可以水平和垂直聚集在微通道的中心。嵌入了光纤和片上微透镜，用于激发和检测单细胞。通过对临床血液样本中的白细胞进行分类来验证微流控芯片。