Key message

Morphological, agronomical and physiological performance of clonal coconut palms, and the proximate composition (kernel), sugar profile (coconut water) were either similar or superior to the seed germinated palms. Genetic stability of regenerated coconut plants was confirmed using simple sequence repeat (SSR) markers.

Abstract

Micropropagation of coconut provides a way for a large-scale propagation of elite coconut cultivars for both local and international markets. The use of micropropagated plants in coconut industry depends on the evidence to prove that such materials are not divergent from ordinary plant materials produced through conventional methods. The present study was designed to evaluate the genetic fidelity and various morphological, agronomical, physiological and fruit nutrient component parameters of similar aged micropropagated and seed-derived hybrid coconut CRIC65, after 12 years of growth in the field. Molecular characterization using simple sequence repeat markers (SSR) provides assurance of genetic fidelity of regenerated palms by scoring identical alleles at the genomic SSR loci tested in the study. Sugar profiles of coconut water and proximate compositions of the coconut kernel showed no significant differences between micropropagated and seed-propagated progenies. Micropropagated coconut palms had an overall physiological performance similar to seed-derived palms. While the majority of the fruit components were statistically similar between the micropropagated and seed-derived progenies, a few economically important components of the fruits such as nut weight, kernel fresh weight and kernel dry weight were superior in micropropagated plants. This suggests that the field performance of the clones of CRI65 derived through micropropagation to be comparative or superior depending on the criteria used in the parental selection of hybrid production. Accordingly, in addition to the multiplication of clonal material of genetically improved coconut hybrids micropropagation techniques facilitate the improvement of the clonal progeny by selecting superior mother palms for micropropagation.

中文翻译：

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微繁殖和种子种植椰子 (Cocos nucifera L.) 棕榈树的生理、生化和分子评估

关键信息

无性系椰子树的形态学、农艺学和生理学性能以及近似成分（仁）、糖分（椰子水）与种子发芽的棕榈树相似或优越。使用简单序列重复 (SSR) 标记确认再生椰子植物的遗传稳定性。

抽象的

椰子的微繁殖为本地和国际市场的优质椰子品种大规模繁殖提供了一种途径。微繁植物在椰子工业中的使用取决于证明此类材料与通过常规方法生产的普通植物材料没有区别的证据。本研究旨在评估经过 12 年田间生长后的类似老化微繁和种子来源的杂交椰子 CRIC65 的遗传保真度和各种形态、农艺、生理和果实营养成分参数。使用简单序列重复标记 (SSR) 的分子表征通过对研究中测试的基因组 SSR 位点的相同等位基因进行评分来保证再生棕榈树的遗传保真度。椰子水的糖谱和椰子仁的近似组成显示微繁殖和种子繁殖后代之间没有显着差异。微繁殖的椰子树具有与种子衍生的棕榈树相似的整体生理性能。虽然微繁后代和种子衍生后代的大多数果实成分在统计上相似，但果实的一些经济上重要的成分，如坚果重量、籽粒鲜重和籽粒干重在微繁植株中更为优越。这表明根据杂交生产的亲本选择中使用的标准，通过微繁殖获得的 CRI65 克隆的田间性能具有可比性或优越性。因此，