Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3-L1 preadipocytes,Journal of Food Biochemistry

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Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3-L1 preadipocytes
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-09 , DOI: 10.1111/jfbc.13896
Won-Ju Kim ₁ , Hyung-Seok Yu ₁ , Won-Young Bae ₁ , Kyung Yuk Ko ₂ , Kyung-Hoon Chang ₃ , Na-Kyoung Lee ₁ , Hyun-Dong Paik ₁

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Herbs have been of interest to treat diseases, including obesity, owing to their various bioactive constituents that exhibit therapeutic and prophylactic properties. The present study examined the anti-adipogenic effects and mechanisms of Chrysanthemum indicum aqueous extract (CAE) in 3T3-L1 preadipocytes. CAE comprises 1,3-dicaffeoylquinic acid, chlorogenic acid, kaempferol-3-O-glucoside, caffeic acid, and apigenin, which were corresponded with previous reports. CAE inhibited the accumulation of lipid droplets and significantly alleviated the expression of lipogenesis- and adipogenesis-associated biomarkers. Treatment with CAE inhibited the mitotic clonal expansion (MCE), corroborated by cell cycle arrest at the G₀/G₁ phase, and mitigated the expression of cell cycle progression-associated proteins and in addition to phosphorylation of MCE-promoting transcription factors. Moreover, CAE downregulated the activation of Akt and extracellular signal-regulated kinase 1/2 signaling pathways. In summary, CAE facilitates adipogenic inhibition during the early phase of differentiation, especially MCE, and its phenolic compounds can contribute to its anti-obesogenic properties.

中文翻译：

菊花通过抑制 3T3-L1 前脂肪细胞的有丝分裂克隆扩增来抑制脂肪生成

草药因其具有治疗和预防特性的各种生物活性成分而被用于治疗疾病，包括肥胖症。本研究研究了菊花水提取物 (CAE) 在 3T3-L1 前脂肪细胞中的抗脂肪生成作用和机制。CAE 包含 1,3-二咖啡酰奎宁酸、绿原酸、山奈酚-3- O-葡萄糖苷、咖啡酸和芹菜素，这与之前的报道一致。CAE 抑制脂滴的积累并显着减轻脂肪生成和脂肪生成相关生物标志物的表达。CAE 治疗抑制了有丝分裂克隆扩增 (MCE)，细胞周期停滞在 G ₀ /G ₁证实了这一点阶段，并减轻细胞周期进程相关蛋白的表达，以及 MCE 促进转录因子的磷酸化。此外，CAE 下调 Akt 和细胞外信号调节激酶 1/2 信号通路的激活。总之，CAE 在分化的早期阶段促进脂肪生成抑制，尤其是 MCE，其酚类化合物有助于其抗肥胖特性。

更新日期：2021-09-06

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