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Pretreatment of outer membrane vesicle and subsequent infection with influenza virus induces a long-lasting adaptive immune response against broad subtypes of influenza virus
Microbes and Infection ( IF 2.6 ) Pub Date : 2021-08-09 , DOI: 10.1016/j.micinf.2021.104878
Chang-Ung Kim 1 , Sukyeong Eo 2 , Pureum Lee 3 , Sang-Hyun Kim 4 , Young Sang Kim 5 , Doo-Jin Kim 6
Affiliation  

Influenza is an acute respiratory disease and a global health problem. Although influenza vaccines are commercially available, frequent antigenic changes in hemagglutinin might render them less effective or unavailable. We previously reported that modified outer membrane vesicle (fmOMV) provided immediate and robust protective immunity against various subtypes of influenza virus. However, the effect was transient because it was innate immunity-dependent. In this study, we investigated the effects of consecutive administration of fmOMV and influenza virus on the adaptive immune response and long-term protective immunity against influenza virus. When the mice were pretreated with fmOMV and subsequently infected with influenza virus, strong influenza-specific antibody and T cell responses were induced in both systemic and lung mucosal compartments without pathogenic symptoms. Upon the secondary viral challenge at week 4, the mice given fmOMV and influenza virus exhibited almost complete protection against homologous and heterologous viral challenge. More importantly, this strong protective immunity lasted up to 18 weeks after the first infection. These results show that pretreatment with fmOMV and subsequent infection with influenza virus efficiently induces broad and long-lasting protective immunity against various virus subtypes, suggesting a novel antiviral strategy against newly-emerging viral diseases without suitable vaccines or therapeutics.



中文翻译:

外膜囊泡的预处理和随后的流感病毒感染诱导针对流感病毒广泛亚型的长期适应性免疫反应

流感是一种急性呼吸道疾病,是一个全球性的健康问题。尽管流感疫苗是市售的,但血凝素的频繁抗原变化可能会使它们的效果降低或无法获得。我们之前报道了修饰的外膜囊泡 (fmOMV) 提供了针对各种流感病毒亚型的即时和强大的保护性免疫。然而,这种影响是短暂的,因为它依赖于先天免疫。在这项研究中,我们研究了连续施用 fmOMV 和流感病毒对适应性免疫反应和针对流感病毒的长期保护性免疫的影响。当小鼠用 fmOMV 预处理并随后感染流感病毒时,在全身和肺粘膜区室均诱导出强流感特异性抗体和 T 细胞反应,而没有致病症状。在第 4 周进行第二次病毒攻击后,给予 fmOMV 和流感病毒的小鼠表现出几乎完全抵抗同源和异源病毒攻击的保护。更重要的是,这种强大的保护性免疫在第一次感染后持续长达 18 周。这些结果表明,用 fmOMV 预处理和随后用流感病毒感染可有效地诱导针对各种病毒亚型的广泛而持久的保护性免疫,这表明在没有合适的疫苗或治疗剂的情况下针对新出现的病毒性疾病的新抗病毒策略。给予 fmOMV 和流感病毒的小鼠对同源和异源病毒攻击表现出几乎完全的保护。更重要的是,这种强大的保护性免疫在第一次感染后持续长达 18 周。这些结果表明,用 fmOMV 预处理和随后用流感病毒感染可有效地诱导针对各种病毒亚型的广泛而持久的保护性免疫,这表明在没有合适的疫苗或治疗剂的情况下针对新出现的病毒性疾病的新抗病毒策略。给予 fmOMV 和流感病毒的小鼠对同源和异源病毒攻击表现出几乎完全的保护。更重要的是,这种强大的保护性免疫在第一次感染后持续长达 18 周。这些结果表明,用 fmOMV 预处理和随后用流感病毒感染可有效地诱导针对各种病毒亚型的广泛而持久的保护性免疫,这表明在没有合适的疫苗或治疗剂的情况下针对新出现的病毒性疾病的新抗病毒策略。

更新日期:2021-08-09
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