Preparation and Characterization of Palladium Derivate-Loaded Micelle Formulation in Vitro as an Innovative Therapy Option against Non-Small Cell Lung Cancer Cells,Chemistry & Biodiversity

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Preparation and Characterization of Palladium Derivate-Loaded Micelle Formulation in Vitro as an Innovative Therapy Option against Non-Small Cell Lung Cancer Cells
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-08-09 , DOI: 10.1002/cbdv.202100402
Merve Erkisa _{1,

2} , Ferda Ari ₁ , Gülay Büyükköroğlu ₃ , Behiye Şenel ₃ , Veysel Turan Yilmaz ₄ , Engin Ulukaya ₅

Affiliation

Nanoparticles have been used in cancer treatments to target tumor and reduce side effects. In this study, we aimed to increase the effectiveness of palladium(II) complex [PdCl(terpy)](sac) ⋅ 2H₂O, which previously showed anticancer potential, by preparing the nanoparticle formulation. An inhalable micellar dispersion containing a palladium(II) complex (PdNP) was prepared and its physicochemical characteristics were evaluated using in vitro tests. Morphology, size and surface charges of particle and loading/encapsulation efficiency of PdNP were analyzed by scanning electron microscopy, zeta sizer and inductively coupled plasma mass spectrometry while aerosol properties of PdNP were measured by the next generation impactor. A549 and H1299 non-small lung cancer cell types were used for cytotoxicity using SRB and ATP assays. Fluorescent staining and M30 antigen assay were carried out for cell death evaluation. Apoptosis was confirmed by flow cytometry analyses.

中文翻译：

钯衍生物负载胶束制剂的体外制备和表征作为针对非小细胞肺癌细胞的创新治疗选择

纳米粒子已被用于癌症治疗以靶向肿瘤并减少副作用。在这项研究中，我们旨在通过制备纳米颗粒制剂来提高钯 (II) 络合物 [PdCl(terpy)](sac) ⋅ 2H ₂ O的有效性，该络合物先前已显示出抗癌潜力。制备了一种含有钯 (II) 络合物 (PdNP) 的可吸入胶束分散体，并在体外评估了其理化特性。测试。通过扫描电子显微镜、zeta sizer 和电感耦合等离子体质谱法分析颗粒的形态、尺寸和表面电荷以及 PdNP 的负载/封装效率，同时通过下一代撞击器测量 PdNP 的气溶胶特性。A549 和 H1299 非小肺癌细胞类型用于使用 SRB 和 ATP 测定的细胞毒性。进行荧光染色和 M30 抗原测定以评估细胞死亡。通过流式细胞术分析证实了细胞凋亡。

更新日期：2021-09-13

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