Abstract

The formation of biofilms is a survival strategy employed by bacteria to help protect them from changing or unfavourable environments. In this research, 319 genes which govern biofilm formation in V. parahaemolyticus, as reported in 1,625 publications, were analysed using protein-protein-interaction (PPI) network analysis. CsrA was identified as a motility-sessility switch and biofilm formation regulator. Through robust rank aggregation (RRA) analysis of GSE65340, the generation of viable but non-culturable (VBNC) cells that may enhance cell tolerance to stress, was found to be associated with the TCA cycle and carbon metabolism biological pathways. The finding that CsrA is likely to play a role in the development of VBNC cells improves understanding of the molecular mechanisms of VBNC formation in V. parahaemolyticus and contributes to on-going efforts to reduce the hazard posed by this foodborne pathogen.

摘要

生物膜的形成是细菌用来帮助保护它们免受变化或不利环境影响的生存策略。在这项研究中，使用蛋白质-蛋白质相互作用 (PPI) 网络分析对 1,625 篇出版物中报道的319 个控制副溶血性弧菌生物膜形成的基因进行了分析。CsrA 被确定为动力-固着性开关和生物膜形成调节剂。通过对 GSE65340 的稳健秩聚合 (RRA) 分析，发现可增强细胞对压力的耐受性的可行但不可培养 (VBNC) 细胞的产生与 TCA 循环和碳代谢生物学途径有关。CsrA 可能在 VBNC 细胞的发育中发挥作用的发现提高了对 VBNC 形成的分子机制的理解V. parahaemolyticus并有助于持续努力减少这种食源性病原体造成的危害。