Hydrogen-rich water reduced oxidative stress and renal fibrosis in rats with unilateral ureteral obstruction,Pediatric Research

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Hydrogen-rich water reduced oxidative stress and renal fibrosis in rats with unilateral ureteral obstruction
Pediatric Research ( IF 3.1 ) Pub Date : 2021-08-07 , DOI: 10.1038/s41390-021-01648-7
Akira Mizutani ₁ , Amane Endo ₁ , Masahito Saito ₁ , Taichi Hara ₁ , Mayu Nakagawa ₁ , Koji Sakuraya ₁ , Yayoi Murano ₁ , Naoto Nishizaki ₂ , Daishi Hirano ₃ , Shuichiro Fujinaga ₄ , Yoshiyuki Ohtomo ₅ , Toshiaki Shimizu ₁

Affiliation

Background

Congenital obstructive nephropathy (CKD) is commonly implicated in the pathophysiology of chronic kidney disease occurring in the pediatric and adolescent age groups and the release of reactive oxygen species contribute to the worsening of renal fibrosis. Molecular hydrogen (H₂) protects against tissue injury by reducing oxidative stress. We evaluated the efficacy of oral H₂-rich water (HW) intake in preventing unilateral ureteral obstruction (UUO)-induced renal injury in rats.

Methods

Male Sprague–Dawley UUO or control rats were administered with distilled water (DW) or HW for 2 weeks post-surgery. Histopathological and immunohistochemical analyses of kidney samples were performed.

Results

Histological changes were not apparent in the sham-operated kidneys. However, UUO kidneys were found to have widened interstitial spaces and tubular dilatation. Compared with the UUO + DW group, HW administration attenuated tubulointerstitial injury and reduced interstitial fibrotic area, causing a substantial decline in the frequency of α-SMA-, ED-1-, and TGF-β1-positive cells in the UUO + HW group. The decrease in the klotho mRNA expression in the UUO + HW group was less pronounced than that in the UUO + DW group.

Conclusion

Oral HW intake reduced oxidative stress and prevented interstitial fibrosis in UUO kidneys, potentially involving klotho in the underlying mechanism.

Impact

Oral intake of hydrogen-rich water (HW) can reduce oxidative stress and suppress interstitial fibrosis in unilateral ureteral obstruction-induced renal injury in rats.
This mechanism possibly involves klotho, which is known for its antiaging roles.
The association between molecular hydrogen and klotho in renal fibrosis is well known; this is the first report on the association in a unilateral ureteral obstruction model.
Drinking HW is a safe and convenient treatment for oxidative stress-induced pathologies, without side effects.
As a prospect for future research, oral HW intake to treat oxidative stress may improve renal fibrosis in congenital obstructive nephropathy.

中文翻译：

富氢水可减轻单侧输尿管梗阻大鼠的氧化应激和肾纤维化

背景

先天性阻塞性肾病 (CKD) 通常与发生在儿童和青少年年龄组的慢性肾病的病理生理学有关，并且活性氧的释放会导致肾纤维化的恶化。分子氢 (H ₂ ) 通过减少氧化应激来防止组织损伤。我们评估了口服富含 H ₂的水 (HW) 在预防大鼠单侧输尿管梗阻 (UUO) 引起的肾损伤中的功效。

方法

雄性 Sprague-Dawley UUO 或对照大鼠在术后 2 周用蒸馏水 (DW) 或 HW 给药。对肾脏样品进行组织病理学和免疫组织化学分析。

结果

假手术肾脏的组织学变化不明显。然而，发现 UUO 肾脏具有扩大的间质空间和肾小管扩张。与 UUO + DW 组相比，HW 给药减轻了肾小管间质损伤并减少了间质纤维化面积，导致 UUO + HW 组中 α-SMA-、ED-1-和 TGF-β1 阳性细胞的频率显着下降. UUO + HW 组中klotho mRNA 表达的降低不如 UUO + DW 组明显。