Inflammatory cytokine responses in children with asymptomatic malaria infection living in rural, semi-urban and urban areas in south-eastern Gabon,Clinical & Experimental Immunology

当前位置： X-MOL 学术 › Clin. Exp. Immunol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Inflammatory cytokine responses in children with asymptomatic malaria infection living in rural, semi-urban and urban areas in south-eastern Gabon
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2021-08-07 , DOI: 10.1111/cei.13653
Chérone Nancy Mbani Mpega Ntigui _{1,

2} , Sandrine Lydie Oyegue‐Liabagui _{1,

2} , Lady Charlene Kouna ₁ , Karl Roméo Imboumy ₁ , Nathalie Pernelle Tsafack Tegomo ₁ , Alain Prince Okouga ₁ , Seinnat Ontoua ₁ , Jean‐Bernard Lekana‐Douki _{1,

3}

Affiliation

Cytokines are soluble mediators of the immune response, and their evolution influences the disease outcome. Gaining knowledge on cytokines has become important, as they can constitute biomarkers allowing the diagnosis of malaria and preventing severe forms of the disease. Here, we investigated 10 cytokines and their circulating levels in asymptomatic Gabonese children with Plasmodium falciparum infection living in urban, semi-urban and rural areas. Blood samples were collected from 273 schoolchildren (153 uninfected and 120 infected) aged 6 to 192 months. Hematological parameters were determined and P. falciparum diagnosis was performed using a rapid diagnosis test, microscopy and nested polymerase chain reaction (PCR). Plasma pro- [interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-12p70, IL-17A and IL-22] and anti-inflammatory [IL-10, IL-4, IL-13 and transforming growth factor (TGF)-β] cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA) and compared between asymptomatic-infected and uninfected children. Results revealed that without distinction of area, IL-10 and IL-6 levels were higher in infected compared to uninfected children; however, the pro- and anti-inflammatory ratios (IL-6/IL-10 and TNF-α/IL-10) were similar. Furthermore, with area distinction significantly elevated levels of IL-10 in these asymptomatic children were always accompanied by either significantly low or high levels of a proinflammatory cytokine. Also, comparison between asymptomatic-infected children from the three areas showed significantly lower IL-17A, IL-22 and TGF-β levels in urban area compared to semi-urban and rural areas. These results suggest that asymptomatic malaria infections induce significantly high inflammatory cytokine levels without modifying the balanced between pro- and anti-inflammatory cytokines and underline the higher exposure to infections of children in rural areas.

中文翻译：

生活在加蓬东南部农村、半城市和城市地区的无症状疟疾感染儿童的炎症细胞因子反应

细胞因子是免疫反应的可溶性介质，它们的进化会影响疾病的结果。获得有关细胞因子的知识变得很重要，因为它们可以构成生物标志物，从而可以诊断疟疾并预防严重的疾病形式。在这里，我们调查了居住在城市、半城市和农村地区的无症状的加蓬恶性疟原虫感染儿童的 10 种细胞因子及其循环水平。从 6 至 192 个月的 273 名学童（153 名未感染和 120 名感染）中采集了血液样本。测定血液学参数和恶性疟原虫使用快速诊断测试、显微镜检查和巢式聚合酶链反应 (PCR) 进行诊断。血浆原-[干扰素 (IFN)-γ、肿瘤坏死因子 (TNF)-α、白介素 (IL)-6、IL-12p70、IL-17A 和 IL-22] 和抗炎药 [IL-10、IL- 4、IL-13和转化生长因子（TGF）-β]细胞因子水平通过酶联免疫吸附试验（ELISA）测定并比较无症状-受感染和未受感染的儿童。结果显示，不区分地区，感染儿童的IL-10和IL-6水平高于未感染儿童；然而，促炎和抗炎比率（IL-6/IL-10 和 TNF-α/IL-10）相似。此外，这些无症状儿童的区域差异显着升高的 IL-10 水平总是伴随着显着低或高水平的促炎细胞因子。此外，三个地区的无症状感染儿童之间的比较显示，与半城市和农村地区相比，城市地区的 IL-17A、IL-22 和 TGF-β 水平显着降低。

更新日期：2021-08-07

点击分享查看原文

点击收藏

阅读更多本刊最新论文