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Hypoxia inducible factor 1-α is minimally involved in determining the time domains of the hypoxic ventilatory response in adult zebrafish (Danio rerio)
Respiratory Physiology & Neurobiology ( IF 2.3 ) Pub Date : 2021-08-08 , DOI: 10.1016/j.resp.2021.103774
Milica Mandic 1 , Adrian Bailey 1 , Steve F Perry 1
Affiliation  

In the current study, adult zebrafish (Danio rerio) were exposed to 72 h hypoxia (90 mmHg) to assess the time domains of the hypoxia ventilatory response (HVR) and the consequence on a subsequent more severe (40 mmHg) bout of acute hypoxia. Experiments were performed on wild-type fish and mutants in which one or both paralogs of hypoxia inducible factor-1α (hif-1α) were knocked out. Although there were subtle differences among the wild-type and knockout fish, resting fV was reestablished after 2–8 h of continuous hypoxia in both groups, a striking example of hypoxic ventilatory decline (HVD). When fish were subsequently exposed to more severe hypoxia, a rapid increase in fV was observed, the magnitude of which was independent of genotype or prior exposure history. During recovery, fish that had been exposed to 72 h of 90 mmHg hypoxia exhibited a pronounced undershoot in fV, which was absent in the hif-1α double knockouts. Overall, the results revealed distinct time domains of the HVR in zebrafish that were largely Hif-1α-independent.



中文翻译:

缺氧诱导因子 1-α 在确定成年斑马鱼 (Danio rerio) 缺氧通气反应的时间域中的作用最小

在目前的研究中,成年斑马鱼 ( Danio rerio ) 暴露于 72 小时缺氧 (90 mmHg) 以评估缺氧通气反应 (HVR) 的时域以及随后更严重 (40 mmHg) 急性缺氧发作的后果. 对缺氧诱导因子-1α ( hif-1α ) 的一种或两种旁系同源物被敲除的野生型鱼和突变体进行了实验。虽然野生型鱼和淘汰鱼之间存在细微差异,但两组在持续缺氧 2-8 小时后恢复了静息f V ,这是缺氧通气下降 (HVD) 的一个显着例子。当鱼随后暴露于更严重的缺氧时,f V迅速增加观察到,其大小与基因型或先前的接触史无关。在恢复过程中,暴露于 90 mmHg 缺氧 72 小时的鱼在f V中表现出明显的下冲,这在hif-1α双敲除中不存在。总体而言,结果揭示了斑马鱼中 HVR 的不同时域,这些时域在很大程度上与 Hif-1α 无关。

更新日期:2021-08-13
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