Anaplasma spp. are among the most recognized arthropod-borne infectious agents. Although the novel A. capra has been isolated from wildlife, livestock, and hard ticks from many parts of the world, there is no report regarding the identification of this pathogen from equines and little is known about the epidemiology of A. capra in Equidae. In this study, A. capra was identified in two out of ten blood specimens of wild onagers (Equus hemionus onager) during a routine health check-up in Semnan, Iran by light microscopy and molecular analyses while other pathogens were not detected. First, inclusions on RBC's were observed in two blood smears by light microscopy. Then, the blood specimens of both animals were analyzed by realtime-PCR for Anaplasma, Ehrlichia, and Theileria infections. A 1400 bp sequence of 16S rRNA belonging to Anaplasmataceae and 874 bp fragment for groEL gene for A. capra were amplified in Anaplasma positive samples and sequenced. Preliminary BLAST analysis of sequenced fragments showed high homology to A. capra strains in GenBank database. Finally, nested PCR and restriction enzyme fragment length polymorphism techniques confirmed the pathogen as A. capra. To the best of our knowledge, this study has reported the occurrence of A. capra in wild onagers for the first time and suggests that equines could be infected with this pathogen and act as reservoirs for A. capra.

无形体属。是最受认可的节肢动物传播的传染性病原体之一。虽然新型A. capra已从世界许多地方的野生动物、牲畜和硬蜱中分离出来，但没有关于从马中鉴定这种病原体的报告，对A. capra在Equidae 中的流行病学知之甚少。在这项研究中，A.卡普拉进二出野生弩炮十血标本经鉴定（野驴野驴) 在伊朗塞姆南通过光学显微镜和分子分析进行的例行健康检查中，而其他病原体未检测到。首先，通过光学显微镜在两份血涂片中观察到红细胞上的内含物。然后，通过实时-PCR 分析两只动物的血液样本中的无形体、埃立克体和泰勒虫感染。在无形体阳性样品中扩增了属于无形体科的 16S rRNA 的 1400 bp 序列和山羊A. groEL 基因的 874 bp 片段并测序。测序片段的初步 BLAST 分析显示与A. capra具有高度同源性GenBank 数据库中的菌株。最后，巢式PCR和限制酶片段长度多态性技术证实病原体为A. capra。据我们所知，这项研究首次报道了野兔中A. capra的发生，并表明马可能感染了这种病原体并作为A. capra 的储存库。