ABSTRACT

Fructooligosaccharides (FOS), a prebiotic supplement, can enhance immunological responses, probably through regulation of gastrointestinal microflora. On the other hand, some environmental pollutants have adjuvanticity against allergic reactions/diseases. The purpose of this study was to evaluate the effects of dietary supplementation with FOS on a murine model of enhanced ovalbumin (OVA)-related allergic peritonitis induced by di-(2-ethylhexyl) phthalate (DEHP). C3H/HeN mice were intraperitoneally administered OVA and DEHP, and fed a diet containing 2.5% FOS. Thereafter, several parameters were evaluated. Supplementation with FOS alleviated OVA plus DEHP-related peritoneal inflammation characterized by infiltration of polymorphonuclear leukocytes including neutrophils in the peritoneal cavity. Also, FOS administration declined the number of mast cells of OVA + DEHP treated mice. FOS suppressed the elevated protein level of interleukin-5, eotaxin, and keratinocyte-derived chemoattractant, in the peritoneal lavage fluids. Our results suggest that dietary supplementation with FOS can prevent or ameliorate enhanced allergic peritoneal inflammation induced by DEHP.

摘要

低聚果糖 (FOS) 是一种益生元补充剂，可能通过调节胃肠道微生物群来增强免疫反应。另一方面，一些环境污染物对过敏反应/疾病具有辅助作用。本研究的目的是评估膳食补充 FOS 对由邻苯二甲酸二(2-乙基己基)酯 (DEHP) 诱导的增强型卵清蛋白 (OVA) 相关过敏性腹膜炎小鼠模型的影响。C3H/HeN 小鼠腹腔注射 OVA 和 DEHP，并喂食含有 2.5% FOS 的饮食。此后，评估了几个参数。补充 FOS 减轻了 OVA 和 DEHP 相关的腹膜炎症，其特征是多形核白细胞浸润，包括腹腔中的中性粒细胞。还，FOS 给药降低了 OVA + DEHP 治疗小鼠的肥大细胞数量。FOS 抑制腹膜灌洗液中白细胞介素 5、嗜酸性粒细胞趋化因子和角质形成细胞衍生的化学引诱物的蛋白质水平升高。我们的结果表明，膳食补充果寡糖可以预防或改善由 DEHP 引起的过敏性腹膜炎症。