ABSTRACT

Application of bio-fertilizer with the ability to promote root development in legume plants could be beneficial, and it is vital to find an inoculation method that does not inhibit symbiosis between rhizobium and plants. However, co-inoculation of rhizobia with different microorganisms on legumes generally inhibits interdependency with rhizobia. The present study was conducted to determine the ideal inoculation method of the bio-fertilizer ‘Yume-bio’ containing Bacillus pumilus TUAT1, which has plant growth-promoting activity, without inhibiting rhizobial infection of soybean. Soybean plants were inoculated with Bradyrhizobium diazoefficiens USDA110 during the time of sowing seeds. Then, two types of ‘Yume-bio’ applications were conducted: simultaneous inoculation with the rhizobia (SI) and 1 week after inoculation the rhizobia (I). No inoculation (NI) was used as the control. Four weeks after sowing, biomass dry weight (shoot and root) and nitrogenase activity based on acetylene reduction assay (ARA) both, per plant and nodule weight increased significantly in ‘I’ treatment compared to those in ‘NI.’ However, there were no significant differences between ‘SI’ and ‘NI’ for shoot biomass and ARA per plant. Nodule numbers decreased in ‘SI’ as compared to ‘NI,’ while they were not different between ‘I’ and ‘NI.’ This study suggests that simultaneous inoculation of ‘Yume-bio’ and rhizobia inhibits nodule development, while inoculation of ‘Yume-bio’ 1 week after inoculation of rhizobia is ideal for promoting soybean growth without inhibiting rhizobium infection in soybean.

摘要

应用具有促进豆科植物根系发育能力的生物肥料可能是有益的，找到一种不抑制根瘤菌与植物共生的接种方法至关重要。然而，根瘤菌与不同微生物在豆类上的共同接种通常会抑制与根瘤菌的相互依赖性。本研究旨在确定含有短小芽孢杆菌TUAT1的生物肥料“Yume-bio”的理想接种方法，该生物肥料具有促进植物生长的活性，同时不抑制大豆根瘤菌感染。用重氮缓生菌接种大豆植株播种期间 USDA110。然后，进行了两种类型的“Yume-bio”应用：同时接种根瘤菌 (SI) 和接种根瘤菌 1 周后 (I)。不使用接种 (NI) 作为对照。播种后 4 周，与 'NI' 处理相比，'I' 处理的生物量干重（茎和根）和固氮酶活性（基于乙炔还原试验（ARA））、单株和根瘤重量均显着增加。然而，每株植物的枝条生物量和 ARA 的“SI”和“NI”之间没有显着差异。与“NI”相比，“SI”中的结节数量减少，而“I”和“NI”之间没有差异。这项研究表明，同时接种“Yume-bio”和根瘤菌可抑制根瘤的发育，