Objective

This study evaluated the cytotoxic and estrogenic effects of dust and eluates released into simulated wastewater after grinding of dental resin-based materials.

Methods

Four materials were used: ceram.x® universal, Filtek™ Supreme XTE, Lava™ Ultimate and Core-X™ flow. From each composite material, samples (5 × 2 mm, n = 50) were prepared according to the manufacturers’ instructions. Lava™ Ultimate was used as blocks. All samples were ground to dust with a diamond bur (106 μm) and suspended in distilled water at 60 mg/mL. After storage for 72 h, the suspensions were separated into a soluble (eluate) and a particulate (dust) fraction. Eluates and dusts were evaluated for inhibition of Vibrio fischeri bioluminescence and cytotoxicity on human A549 lung cells (WST-1-Assay). The estrogenic activity was assessed by YES-Assay using Saccharomyces cerevisiae. Additionally, dental monomers (BisGMA, BisEMA, UDMA, TEGDMA, HEMA) and Bisphenol A were investigated.

Results

All eluates showed inhibition of V. fischeri bioluminescence at concentrations above 1.1 mg/mL (p < 0.05). The activity of the eluates of ceram.x® universal and Filtek™ Supreme XTE was significantly higher than Lava™ Ultimate and Core-X™ flow (p < 0.05). In the WST-1-Assay, all materials induced cytotoxic effects at concentrations of 0.1 mg/mL (p < 0.05), while no significant differences were detected among them. The tested materials revealed no estrogenic activity. All dental monomers and Bisphenol A showed concentration dependent cytotoxic effects (p < 0.05), whereas only Bisphenol A induced an estrogenic effect (p < 0.01).

Significance

Dust and eluates of resin-based dental materials released into wastewater exert bactericidal and cytotoxic effects in vitro. However, they reveal no estrogenic effect.

中文翻译：

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树脂基材料研磨后模拟牙科废水中的细胞毒性和雌激素

客观的

本研究评估了研磨牙科树脂基材料后释放到模拟废水中的灰尘和洗脱液的细胞毒性和雌激素作用。

方法

使用了四种材料：ceram.x® Universal、Filtek™ Supreme XTE、Lava™ Ultimate 和 Core-X™ flow。根据制造商的说明，从每种复合材料制备样品（5 × 2 mm，n = 50）。Lava™ Ultimate 被用作块。所有样品都用金刚石车针 (106 μm) 研磨成粉，并以 60 mg/mL 的浓度悬浮在蒸馏水中。储存 72 小时后，悬浮液被分离成可溶（洗脱液）和颗粒（粉尘）部分。评估洗脱液和粉尘对费氏弧菌生物发光的抑制和对人 A549 肺细胞的细胞毒性 (WST-1-Assay)。雌激素活性通过使用酿酒酵母的YES-Assay 进行评估。此外，还研究了牙科单体（BisGMA、BisEMA、UDMA、TEGDMA、HEMA）和双酚 A。

结果

所有洗脱液均显示出对V 的抑制。fischeri生物发光浓度高于 1.1 mg/mL (p < 0.05)。ceram.x® Universal 和 Filtek™ Supreme XTE 洗脱液的活性显着高于 Lava™ Ultimate 和 Core-X™ flow（p < 0.05）。在 WST-1-Assay 中，所有材料均在 0.1 mg/mL (p < 0.05) 的浓度下诱导细胞毒性作用，但在它们之间没有检测到显着差异。测试材料显示没有雌激素活性。所有牙科单体和双酚 A 都显示出浓度依赖性细胞毒性作用 (p < 0.05)，而只有双酚 A 诱导雌激素作用 (p < 0.01)。

意义

释放到废水中的树脂基牙科材料的灰尘和洗脱液在体外具有杀菌和细胞毒性作用。然而，它们没有显示出雌激素作用。