Carbazate-modified cross-linked dextran microparticles suppress the progression of osteoarthritis by ROS scavenging,Biomaterials Science

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Carbazate-modified cross-linked dextran microparticles suppress the progression of osteoarthritis by ROS scavenging
Biomaterials Science ( IF 5.8 ) Pub Date : 2021-07-13 , DOI: 10.1039/d1bm00743b
Yanfeng Ding _{1,

2} , Zhimin Li _{2,

3} , Wenwen Hu ₄ , Xianjing Feng ₅ , Ying Chen ₄ , Guohua Yan _{2,

6} , Yonglin Wang ₂ , Bo Zhu _{1,

2} , Wei Yao _{1,

2} , Li Zheng _{1,

2} , Maolin He ₃ , Ming Gao _{1,

2} , Jinmin Zhao _{1,

2,

6,

7}

Affiliation

A series of modified polysaccharide microparticles have been fabricated and their potential application for scavenging reactive oxygen species (ROS) and their derivatives to achieve osteoarthritis (OA) treatment has been explored. These microparticles were cross-linked dextran (Sephadex) with different carbazate substitution ratios determined by the TNBS assay and elemental analysis. It has been demonstrated that they could effectively scavenge carbonylated proteins and ROS including hydroxyl radicals (˙OH), superoxide anions (˙O₂⁻) and H₂O₂ and their derivatives with high efficiency, improve the viability of H₂O₂-treated chondrocytes by reducing their ROS levels, as well as lower their inflammatory factors. The above ability of antioxidation and inflammation resistance improved with the increase of carbazate substitution ratio. Significantly, this work provided the proof that modified Sephadex successfully alleviated the deterioration of cartilage and the progression of OA in vivo. The proposed microparticles showed a very promising capability for reducing ROS levels and further treating OA.

中文翻译：

氨基甲酸酯修饰的交联葡聚糖微粒通过清除 ROS 抑制骨关节炎的进展

已经制备了一系列改性多糖微粒，并探索了它们在清除活性氧 (ROS) 及其衍生物以实现骨关节炎 (OA) 治疗方面的潜在应用。这些微粒是交联葡聚糖 (Sephadex)，具有通过 TNBS 测定和元素分析确定的不同氨基甲酸酯取代率。已证明它们可以高效清除羰基化蛋白质和ROS，包括羟基自由基（˙OH）、超氧阴离子（˙O ₂⁻）和H ₂ O _{2及其衍生物，提高H}₂ O ₂的活力-通过降低其 ROS 水平以及降低其炎症因子来处理软骨细胞。上述抗氧化和抗炎能力随着氨基甲酸取代率的增加而提高。值得注意的是，这项工作提供了修饰后的 Sephadex 成功缓解体内软骨退化和 OA 进展的证据。所提出的微粒显示出非常有前途的降低 ROS 水平和进一步治疗 OA 的能力。

更新日期：2021-08-09

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