Staphylococcus aureus is an important human opportunistic pathogen that can have a major influence on public health. Here, we aimed to evaluate different aspects of the immune response to a novel multi-epitope fusion protein (HMS) based on Hla_H35L, MntC, and SACOL0723 proteins in comparison to the individual antigens. For this purpose, specific total IgG, IgG1, and IgG2a isotypes and the cytokines related to Th1, Th2, and Th17 were assessed. The Bio-efficiency of the fusion protein was evaluated by opsonic killing activity. The HMS fusion protein elicited a high specific IgG level and also induced a higher level of Th1, Th2, and Th17-related cytokines which were more polarized towards the Th1 and Th17 compared to individual antigens. The HMS-specific antisera also significantly promoted phagocytosis of S. aureus COL strain by mouse macrophages. In conclusion, the fusion protein might be an effective vaccine for potential protective immunity against a lethal infection of S. aureus in mice.

金黄色葡萄球菌是一种重要的人类机会性病原体，可对公众健康产生重大影响。在这里，我们旨在评估对基于 Hla _H35L的新型多表位融合蛋白 (HMS) 的免疫反应的不同方面、MntC 和 SACOL0723 蛋白与单个抗原的比较。为此，评估了特定的总 IgG、IgG1 和 IgG2a 同种型以及与 Th1、Th2 和 Th17 相关的细胞因子。通过调理性杀伤活性评价融合蛋白的生物效率。HMS 融合蛋白引发了高特异性 IgG 水平，还诱导了更高水平的 Th1、Th2 和 Th17 相关细胞因子，与单个抗原相比，这些细胞因子更倾向于 Th1 和 Th17。HMS 特异性抗血清还显着促进了小鼠巨噬细胞对金黄色葡萄球菌COL 菌株的吞噬作用。总之，融合蛋白可能是一种有效的疫苗，可针对小鼠金黄色葡萄球菌的致命感染提供潜在的保护性免疫。