In this report, we investigate whether the acute effects of different ethanol (EtOH) concentrations are sex-dependent in zebrafish subjected to the open field test (OFT) with the influence of a non-familiar object. Male and female zebrafish were separated into four groups and exposed to EtOH (0%, 0.25%, 0.5%, or 1.0% v/v) for 1 h. Fish were tested individually in the OFT, in which tank was divided into three areas: periphery, intermediate, and center area. An object (black sphere; diameter: 1 cm) was placed in the center of the tank and behaviors were recorded for 5 min. At the baseline, females had a distinct exploratory activity and interaction pattern with the object, reflecting a more anxious and shyer behavior in relation to males. Females exposed to 0.5% EtOH performed more rapid investigation to the object than males, while 1.0% EtOH reduced locomotion in both sexes and increased immobility only in males.

Principal component analyses revealed that anxiety-like behaviors, exploratory activity, and locomotion were the components that most accounted for total variances.

Collectively, our novel findings show the existence of a sex-dependent effect in the zebrafish models acutely exposed to EtOH tested in the OFT with a non-familiar object.

在本报告中，我们调查了不同乙醇 (EtOH) 浓度的急性影响是否对斑马鱼进行了非熟悉物体影响的露天试验 (OFT) 的性别依赖性。将雄性和雌性斑马鱼分成四组，并暴露于乙醇（0%、0.25%、0.5% 或 1.0% v/v）中 1 小时。在 OFT 中对鱼进行了单独测试，其中鱼缸分为三个区域：外围、中间和中心区域。将一个物体（黑色球体；直径：1 厘米）放置在水箱的中心，并记录其行为 5 分钟。在基线时，女性对物体有明显的探索活动和互动模式，反映了与男性相比更焦虑和更害羞的行为。暴露于 0.5% EtOH 的女性对物体的调查速度比男性快，而 1.

主成分分析显示，焦虑样行为、探索性活动和运动是占总方差的最大成分。

总的来说，我们的新发现表明，在 OFT 中用不熟悉的物体测试的急性暴露于 EtOH 的斑马鱼模型中存在性别依赖性效应。