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Effects of spermine on the proliferation and migration of porcine intestinal epithelial cells
Animal Biotechnology ( IF 1.7 ) Pub Date : 2021-08-08 , DOI: 10.1080/10495398.2021.1955699
Xiaomei Xu 1, 2, 3 , Guangmang Liu 1, 2, 3 , Gang Jia 1, 2, 3 , Hua Zhao 1, 2, 3 , Xiaoling Chen 1, 2, 3 , Caimei Wu 1, 2, 3 , Jing Wang 4
Affiliation  

Abstract

Whether spermine promotes the repair of porcine intestinal epithelium damage through Ras-related C3 botulinum toxin substrate 1 (Rac1)/phospholipase C-γ1 (PLC-γ1) signaling remains unclear. The current study investigated the effects of spermine addition on the proliferation and migration of IPEC-J2 cells and the effects of Rac1/PLC-γ1 signaling on cell migration. We showed that the inhibitors of Rac1 (NSC-23766) and PLC-γ1 (U73122) reduced cell migration and decreased the protein levels of Rac1 and PLC-γ1 in the cells. Moreover, spermine promoted the proliferation and migration of the IPEC-J2 cells, that is, 1 µM spermine exhibited the best effect, and spermine treatment increased the protein levels of Rac1 and PLC-γ1. Further experiments showed that spermine treatment increased cell migration and enhanced Rac1 and PLC-γ1 protein levels, compared with NSC-23766 and U73122 treatments with spermine. In conclusion, spermine treatment promoted the repair of damaged porcine intestinal epithelium by accelerating cell proliferation and migration mediated by Rac1/PLC-γ1 signaling.



中文翻译:

精胺对猪肠上皮细胞增殖和迁移的影响

摘要

精胺是否通过Ras相关的C3肉毒毒素底物1(Rac1)/磷脂酶C-γ1(PLC-γ1)信号通路促进猪肠上皮损伤修复尚不清楚。目前的研究调查了添加精胺对 IPEC-J2 细胞增殖和迁移的影响以及 Rac1/PLC-γ1 信号对细胞迁移的影响。我们发现 Rac1 (NSC-23766) 和 PLC-γ1 (U73122) 的抑制剂减少了细胞迁移并降低了细胞中 Rac1 和 PLC-γ1 的蛋白质水平。此外,精胺促进IPEC-J2细胞的增殖和迁移,即1μM精胺表现出最佳效果,精胺处理增加了Rac1和PLC-γ1的蛋白水平。进一步的实验表明,精胺处理增加了细胞迁移并提高了 Rac1 和 PLC-γ1 蛋白水平,与用精胺处理的 NSC-23766 和 U73122 相比。总之,精胺处理通过加速由 Rac1/PLC-γ1 信号介导的细胞增殖和迁移来促进受损猪肠上皮细胞的修复。

更新日期:2021-08-08
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