Abstract

Whether spermine promotes the repair of porcine intestinal epithelium damage through Ras-related C3 botulinum toxin substrate 1 (Rac1)/phospholipase C-γ1 (PLC-γ1) signaling remains unclear. The current study investigated the effects of spermine addition on the proliferation and migration of IPEC-J2 cells and the effects of Rac1/PLC-γ1 signaling on cell migration. We showed that the inhibitors of Rac1 (NSC-23766) and PLC-γ1 (U73122) reduced cell migration and decreased the protein levels of Rac1 and PLC-γ1 in the cells. Moreover, spermine promoted the proliferation and migration of the IPEC-J2 cells, that is, 1 µM spermine exhibited the best effect, and spermine treatment increased the protein levels of Rac1 and PLC-γ1. Further experiments showed that spermine treatment increased cell migration and enhanced Rac1 and PLC-γ1 protein levels, compared with NSC-23766 and U73122 treatments with spermine. In conclusion, spermine treatment promoted the repair of damaged porcine intestinal epithelium by accelerating cell proliferation and migration mediated by Rac1/PLC-γ1 signaling.

摘要

精胺是否通过Ras相关的C3肉毒毒素底物1（Rac1）/磷脂酶C-γ1（PLC-γ1）信号通路促进猪肠上皮损伤修复尚不清楚。目前的研究调查了添加精胺对 IPEC-J2 细胞增殖和迁移的影响以及 Rac1/PLC-γ1 信号对细胞迁移的影响。我们发现 Rac1 (NSC-23766) 和 PLC-γ1 (U73122) 的抑制剂减少了细胞迁移并降低了细胞中 Rac1 和 PLC-γ1 的蛋白质水平。此外，精胺促进IPEC-J2细胞的增殖和迁移，即1μM精胺表现出最佳效果，精胺处理增加了Rac1和PLC-γ1的蛋白水平。进一步的实验表明，精胺处理增加了细胞迁移并提高了 Rac1 和 PLC-γ1 蛋白水平，与用精胺处理的 NSC-23766 和 U73122 相比。总之，精胺处理通过加速由 Rac1/PLC-γ1 信号介导的细胞增殖和迁移来促进受损猪肠上皮细胞的修复。