Electrochemical methods were used to explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium. We show that the fractional vesicular 5-HT release in BON cells is 80 % compared to previous work in pancreatic beta cells (34 %). The fractional release increased from 80 % in control BON cells to 87 % with 5-HT preincubation and nearly 100 % with the combination of 5-HT and the 5-HT₄ autoreceptor agonist, cisapride. Thus, partial release is the primary mechanism of exocytosis in BON cells, resulting in a variable amount of the vesicular content being released. Factors that control secretion of 5-HT from enterochromaffin cells or BON cells are important as partial release provides a mechanism for development of effective therapeutic strategies to treat gastrointestinal diseases.

中文翻译：

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纳米分析揭示了肠道上皮模型细胞胞吐过程中血清素释放的高比例

电化学方法用于探索人类癌 BON 细胞（一种体外人肠嗜铬细胞模型）中 5-羟色胺 (5-HT) 释放的胞吐性质，以了解肠源性 5-HT 在肠粘膜上皮中的释放机制. 我们表明，与之前在胰腺 β 细胞中的工作 (34%) 相比，BON 细胞中的小泡 5-HT 释放率为 80%。使用 5-HT 预孵育时，释放分数从对照 BON 细胞中的 80% 增加到 87%，而使用 5-HT 和 5-HT ₄的组合时增加到接近 100%自身受体激动剂，西沙必利。因此，部分释放是 BON 细胞胞吐作用的主要机制，导致不同数量的囊泡内容物被释放。控制肠嗜铬细胞或 BON 细胞分泌 5-HT 的因素很重要，因为部分释放为开发治疗胃肠疾病的有效治疗策略提供了机制。