Porcine astrovirus (PAstV) is distributed worldwide and has been reported to cause diarrhea in pigs. PAstV belongs to the family Astroviridae and genus Mamastrovirus. PAstVs are divided into five diverse genotypes (PAstV1-PAstV5) on the basis of phylogenetic analysis of a part of the RNA-dependent RNA polymerase (RdRp) gene and the capsid gene. However, knowledge regarding the clinical significance and molecular characteristics of PAstV in Haryana, India, is limited. In this study, we investigated the presence of PAstV by RT-PCR of the partial RdRp gene in 110 rectal swabs collected from diarrheic pigs in different parts of Haryana, India. Of these, 35 samples (31.8%) tested positive for PAstV, with the highest positivity observed among weaning piglets 3 to 9 weeks of age (47.7%, 21/44), followed by fattening pigs 9 to 24 weeks of age (28.5%, 8/28). Phylogenetic analysis of the partial RdRp gene revealed circulation of four different genotypes (PAstV1, PAstV2, PAstV4, and PAstV5) in Haryana, with PAstV1 being the predominant genotype. To the best of our knowledge, this is the first report of the presence of PAstV1 and PAstV5 in the pig population of India. The PAstV sequences revealed high genetic variability and genetic heterogeneity in a relatively confined area.

猪星状病毒 (PAstV) 分布于世界各地，据报道会导致猪腹泻。PAstV属于星状病毒科和巨乳病毒属。基于部分依赖于 RNA 的 RNA 聚合酶 (RdRp) 基因和衣壳基因的系统发育分析，将 PAstV 分为五种不同的基因型 (PAstV1-PAstV5)。然而，关于印度哈里亚纳邦 PAstV 的临床意义和分子特征的知识是有限的。在这项研究中，我们通过 RT-PCR 对部分 RdRp 基因进行 RT-PCR 研究了 PAstV 的存在，这些基因在从印度哈里亚纳邦不同地区的腹泻猪中收集的 110 份直肠拭子中进行。其中，35 个样本 (31.8%) 的 PAstV 检测呈阳性，在 3 至 9 周龄的断奶仔猪中观察到的阳性率最高 (47.7%, 21/44)，其次是 9 至 24 周龄的育肥猪 (28.5%) , 8/28)。部分 RdRp 基因的系统发育分析揭示了四种不同基因型（PAstV1、PAstV2、PAstV4 和 PAstV5）在哈里亚纳邦的循环，PAstV1 是主要基因型。据我们所知，这是印度猪群中存在 PAstV1 和 PAstV5 的第一份报告。PAstV 序列在相对有限的区域内揭示了高遗传变异性和遗传异质性。