The complete genomic sequence of scorzonera virus A (SCoVA) from a Scorzonera austriaca Willd. plant in South Korea was determined by high-throughput sequencing and confirmed by Sanger sequencing. The SCoVA genome contains 9867 nucleotides, excluding the 3′-terminal poly(A) tail. The SCoVA genome structure is typical of potyviruses and contains a single open reading frame encoding a large putative polyprotein of 3168 amino acids. Pairwise comparison analysis of the complete genome and polyprotein sequences of SCoVA with those of other potyviruses showed that they shared the highest nucleotide and amino acid sequences identity (54.47% and 49.57%, respectively) with those of lettuce mosaic virus (GenBank accession number KJ161185). Phylogenetic analysis of the amino acid sequence of the polyprotein confirmed that SCoVA belongs to the genus Potyvirus. These findings suggest that SCoVA should be considered a novel member of the genus Potyvirus, family Potyviridae.

来自奥地利 Scorzonera病毒 A (SCoVA) 的完整基因组序列威尔德。韩国植物经高通量测序确定，并经桑格测序证实。SCoVA 基因组包含 9867 个核苷酸，不包括 3'-末端 poly(A) 尾。SCoVA 基因组结构是典型的potyviruses 并包含编码3168 个氨基酸的大型推定多聚蛋白的单个开放阅读框。SCoVA的全基因组和多蛋白序列与其他花叶病毒的成对比较分析表明，它们与莴苣花叶病毒（GenBank登录号KJ161185）的核苷酸和氨基酸序列同一性最高（分别为54.47%和49.57%） . 多蛋白氨基酸序列的系统发育分析证实 SCoVA 属于Potyvirus 属. 这些发现表明 SCoVA 应被视为Potyvirus属Potyviridae 的一个新成员。