ABSTRACT

1-deoxynojirimycin (DNJ) has attracted much attention due to its high biological activity, which have favorable anti-diabetic, anti-tumor, and anti-virus effects. In order to explore the effects of maturity, variety, and growth area on the DNJ concentration of mulberry fruit, we examined 146 varieties in this study. First, to explore the influence of maturity, we determined the concentration of DNJ from M. atropurpurea Roxb. and M.wittiorum var. mawa Koidz at different stages of maturity using High Performance Liquid Chromatography (HPLC). On this basis, we next determined the DNJ concentration from 146 mulberry fruits to investigate the effect of variety and growth area, also by HPLC. Our results showed that DNJ existed mainly in the young tissues of mulberry fruit (MS-I). As the mulberry fruit matured, the concentration of DNJ decreased. The DNJ concentrations of 0.005 ~ 0.138 mg/g of fruit weight (FW) accounted for 97.28% of the 146 mulberry fruit varieties. The variety had a significant effect on the DNJ concentration of mulberry fruit, but plant origin did not. Among the varieties, M. atropurpurea Roxb planted in Guangdong had up to 0.1716 ± 0 mg/g FW. This work provides new strategies for the study of DNJ and advances our basic knowledge about DNJ in mulberry fruits.

摘要

1-脱氧野尻霉素（DNJ）因其高生物活性而备受关注，具有良好的抗糖尿病、抗肿瘤和抗病毒作用。为了探索成熟度、品种和生长面积对桑树果实 DNJ 浓度的影响，我们在本研究中检测了 146 个品种。首先，为了探索成熟度的影响，我们确定了M. atropurpurea Roxb中 DNJ 的浓度。和M.witiorum变种。玛娃使用高效液相色谱 (HPLC) 在不同成熟阶段的 Koidz。在此基础上，我们接下来测定了 146 个桑椹果实的 DNJ 浓度，以研究品种和生长面积的影响，同样采用 HPLC。我们的结果表明DNJ主要存在于桑树果实（MS-I）的幼嫩组织中。随着桑树果实的成熟，DNJ的浓度降低。DNJ 浓度为 0.005 ~ 0.138 mg/g 单果重 (FW) 占 146 个桑果品种的 97.28%。品种对桑果的DNJ浓度有显着影响，而植物来源则没有。在品种中，M. atropurpurea广东种植的 Roxb 含量高达 0.1716 ± 0 mg/g FW。这项工作为 DNJ 的研究提供了新的策略，并提高了我们对桑果中 DNJ 的基础知识。