The role of mixoplanktonic algae is increasingly recognised as an important link between primary production and microbial loop processes, particularly in dystrophic systems where staining can both limit light and nutrient availability and increase bacterial productivity. We hypothesised that these three factors would affect the relative abundance of mixotrophs (RA_mixotrophs), with increased light and nutrient availability decreasing RA_mixotrophs and increased bacterial availability increasing RA_mixotrophs. We employed a fully factorial design within near-shore mesocosms in a highly stained lake, with light intensity, bacterial abundance, and phosphorus concentration as treatment variables. Over the initial 3 days of the experiment, when changes were greatest, we found no significant three-way interaction effect among light, phosphorus and nutrients on the RA_mixotrophs. We did find significant two-way interactions between phosphorus and light, and phosphorus and bacteria. RA_mixotrophs was decreased by the addition of phosphorus whether light was added or not, but light only significantly deceased RA_mixotrophs when phosphorus was not added. Phosphorus concentration was also found to decrease RA_mixotrophs whether bacterial food was supplemented or not, but bacteria increased RA_mixotrophs only in mesocosms without phosphorus additions. Collectively, our results indicated that phosphorus concentrations may be the primary driver of mixoplanktonic phytoplankton dynamics in dystrophic systems.

混合浮游藻类的作用越来越被认为是初级生产和微生物循环过程之间的重要联系，特别是在营养不良的系统中，染色既可以限制光和养分的可用性，又可以提高细菌生产力。我们假设这三个因素会影响mixotrophs（RA的相对丰度_mixotrophs），增加光线和养分的有效性降低RA _mixotrophs，增加细菌利用率的增加RA _mixotrophs. 我们在一个高度染色的湖泊中的近岸中间宇宙中采用了全因子设计，以光强度、细菌丰度和磷浓度作为处理变量。在实验的最初 3 天，当变化最大时，我们发现光、磷和养分之间对 RA_{混合营养菌}没有显着的三向相互作用影响。我们确实发现磷和光、磷和细菌之间存在显着的双向相互作用。RA _mixotrophs通过加入磷光是否加入或没有，但光只显著死者RA的下降_mixotrophs不添加磷时。还发现磷浓度会减少 RA_{混合营养菌}是否补充细菌食物，但细菌仅在不添加磷的_中层环境中增加 RA_{混合营养物}。总的来说，我们的结果表明磷浓度可能是营养不良系统中混合浮游浮游植物动力学的主要驱动因素。