Albizia myriophylla has been used in Thai folk medicine for treating inflammation-related diseases. The wood of this medicinal plant is traditionally used as a single herbal drug in the form of an aqueous decoction and as a component in several Thai herbal formulations for the remedy of fever, sore throat, and aphthous ulcers. This study aimed to evaluate in vivo the anti-inflammatory potential and possible mechanism of action of the standardized wood extract of A. myriophylla as well as to investigate the anti-inflammatory activity and physicochemical properties of the developed herbal gel formulation containing standardized wood extract of A. myriophylla. Results of quantitative HPLC analysis demonstrated that the standardized wood extract of A. myriophylla contained 22.95 mg/g of 8-methoxy-7,3′,4′-trihydroxyflavone, a bioactive marker compound of A. myriophylla. The standardized wood extract of A. myriophylla (1% w/v) exhibited remarkable inhibition (54.4 – 80.3%) in the croton oil model of topical inflammation at all assessment times, comparable to standard indomethacin (55.3 – 63.6%). Real-time quantitative reverse transcription-polymerase chain reaction was performed to clarify the anti-inflammatory mechanism of standardized wood extract of A. myriophylla, and the result showed that this standardized extract decreased lipopolysaccharide-induced nitric oxide synthase mRNA levels in a dose-dependent manner. The developed herbal gel containing standardized wood extract of A. myriophylla (1% w/w) had good physicochemical characteristics and exhibited potent inhibition (51.4 – 77.8%) of inflammation in a rat ear edema model at all assessment times, comparable to indomethacin gel (33.3 – 40.5%). The notable anti-inflammatory activity of standardized wood extract of A. myriophylla and its developed herbal gel formulation indicates their potential application as natural anti-inflammatory agents.

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多叶合欢的标准化木材提取物：其作为抗炎草本凝胶配方中活性成分的潜力

多叶合欢已在泰国民间医学中用于治疗炎症相关疾病。这种药用植物的木材传统上以水煎剂的形式用作单一草药，并作为几种泰国草药制剂的成分用于治疗发烧、喉咙痛和口疮性溃疡。本研究旨在评估多叶金针草标准化木材提取物的体内抗炎潜力和可能的作用机制，以及研究开发的含有标准化木材提取物的草药凝胶制剂的抗炎活性和理化性质。 A. 多叶植物。定量 HPLC 分析结果表明，多叶金针菇标准化木材提取物含有 22.95 mg/g 的 8-methoxy-7,3',4'-trihydroxyflavone，A. myriophylla 的生物活性标记化合物。A. myriophylla 的标准化木材提取物 (1% w/v) 在所有评估时间的局部炎症巴豆油模型中均表现出显着的抑制作用 (54.4 – 80.3%)，与标准消炎痛 (55.3 – 63.6%) 相当。实时定量逆转录-聚合酶链反应阐明了多叶木精标准化提取物的抗炎机制，结果表明该标准化提取物以剂量依赖性降低脂多糖诱导的一氧化氮合酶 mRNA 水平。方式。所开发的草药凝胶含有标准化的多叶木提取物（1% w/w），具有良好的理化特性，并且在所有评估时间都对大鼠耳水肿模型的炎症具有​​有效的抑制作用（51.4 – 77.8%），与消炎痛凝胶（33.3 – 40.5%）相当。A. myriophylla 的标准化木材提取物及其开发的草药凝胶制剂具有显着的抗炎活性，表明它们具有作为天然抗炎剂的潜在应用。