Pigments are compounds with highly diverse structures and wide uses, which production is increasing worldwide. An eco-friendly method of bioproduction is to use the ability of some microorganisms to ferment on renewable carbon sources. Wheat bran (WB) is a cheap and abundant lignocellulosic co-product of low recalcitrance to biological conversion. Microbial candidates with theoretical ability to degrade WB were first preselected using specific databases. The microorganisms were Ashbya gossypii (producing riboflavin), Chitinophaga pinensis (producing flexirubin), Chromobacterium vaccinii (violacein) and Gordonia alkanivorans (carotenoids). Growth was shown for each on minimal salt medium supplemented with WB at 5 g.L⁻¹. Activities of the main enzymes consuming WB were measured, showing leucine amino-peptidase (up to 8.45 IU. mL⁻¹) and β-glucosidase activities (none to 6.44 IU. mL⁻¹). This was coupled to a FTIR (Fourier Transform Infra-Red) study of the WB residues that showed main degradation of the WB protein fraction for C. pinensis, C. vaccinii and G. alkanivorans. Production of the pigments on WB was assessed for all the strains except Ashbya, with values of production reaching up to 1.47 mg.L⁻¹. The polyphasic approach used in this study led to a proof of concept of pigment production from WB as a cheap carbon source.

颜料是具有高度多样化结构和广泛用途的化合物，其产量在世界范围内不断增加。一种生态友好的生物生产方法是利用一些微生物在可再生碳源上发酵的能力。小麦麸皮 (WB) 是一种廉价且丰富的木质纤维素副产品，对生物转化的抵抗力低。首先使用特定数据库预先选择具有降解WB的理论能力的微生物候选物。微生物是棉麻白蜡菌（产生核黄素）、松果壳壳多孢菌（产生柔红素）、牛痘色杆菌（紫罗兰素）和Gordonia alkanivorans（类胡萝卜素）。在补充有 5 gL ^-1 WB 的基本盐培养基上显示了每个的生长. 测量消耗WB的主要酶的活性，显示亮氨酸氨基肽酶（高达8.45 IU.mL ^-1）和β-葡萄糖苷酶活性（无至6.44 IU.mL ^-1）。这与 WB 残基的 FTIR（傅立叶变换红外）研究相结合，该研究表明C. pinensis、C. vaccinii和G. alkanivorans的 WB 蛋白质级分主要降解。对除Ashbya之外的所有菌株在 WB 上的色素产量进行了评估，产量值高达 1.47 mg.L ^-1。本研究中使用的多相方法证明了从 WB 作为廉价碳源生产颜料的概念。