Background: Recent evidence has shown that Solute Carrier Family 39 Member 10 (SLC39A10) promoted tumor progression in several cancer types. The study intended to explore the expression and function of SLC39A10 in hepatocellular carcinoma (HCC).
Methods: Multiple bioinformatics analyses were used to evaluate SLC39A10 expression and potential role in HCC. Quantitative real-time polymerase chain reaction and immunohistochemistry were used to confirm SLC39A10 expression. Intro studies were performed to assess the effects of SLC39A10 on HCC cells proliferation and migration. Furthermore, flow cytometry was conducted to identify its specific function in apoptosis of HCC.
Results: SLC39A10 was significantly over-expressed in HCC samples from both bioinformatic databases and our cohort. Survival analyses suggested patients with high expression of SLC39A10 had poor overall survival and disease-free survival (P-value < 0.01). Further, the expression of SLC39A10 was positively correlated with tumor-infiltrating lymphocytes and some immune checkpoints like CTLA4, TIM3 and TGFB1. In HCC cell lines, SLC39A10 knockdown inhibited cells proliferation and migration, but promoted apoptosis.
Conclusion: An increased SLC39A10 expression was found and served as an unfavorable indicator of survival in HCC. Further studies suggested SLC39A10 promotes tumor aggressiveness and may provide a novel target for HCC therapy.



中文翻译：

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SLC39A10 上调可预测预后不良、促进增殖和迁移，并与肝细胞癌的免疫浸润相关

背景：最近的证据表明，溶质载体家族 39 成员 10 (SLC39A10) 促进了几种癌症类型的肿瘤进展。本研究旨在探讨 SLC39A10 在肝细胞癌 (HCC) 中的表达和功能。
方法：使用多种生物信息学分析评估 SLC39A10 在 HCC 中的表达和潜在作用。定量实时聚合酶链反应和免疫组织化学用于确认 SLC39A10 表达。进行了介绍性研究以评估 SLC39A10 对 HCC 细胞增殖和迁移的影响。此外，进行流式细胞术以确定其在HCC细胞凋亡中的特定功能。
结果：SLC39A10 在来自生物信息学数据库和我们的队列的 HCC 样本中显着过表达。生存分析表明，SLC39A10 高表达患者的总生存期和无病生存期较差（P 值 < 0.01）。此外，SLC39A10 的表达与肿瘤浸润淋巴细胞和一些免疫检查点如 CTLA4、TIM3 和 TGFB1 呈正相关。在 HCC 细胞系中，SLC39A10 敲低抑制细胞增殖和迁移，但促进细胞凋亡。
结论：发现增加的 SLC39A10 表达并作为 HCC 存活的不利指标。进一步的研究表明 SLC39A10 可促进肿瘤侵袭性，并可能为 HCC 治疗提供新的靶点。