Abstract

In this study, actinobacteria isolated from Sarıkum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I – PKS-II – NRPS genes were identified ın 17 isolates.

摘要

在这项研究中，对从 Sarıkum 湖沉积物中分离的放线菌进行了表征（Sinop-Turkey）。使用 10 种选择性培养基和稀释接种法共分离出 48 种放线菌。根据16S rRNA基因测序的系统发生分析表明，分离的菌株的40％属于的广泛分布属霉菌，36％属于属孢的菌株的24％属于稀有属如红球菌属，Plantactinospora，Nonomuraea、放线菌和链孢子囊菌。大多数分离菌株属于链霉菌属(40%) 和两个分离株可能是新物种。所有分离株都进行了抗菌活性测试；只有 12 个分离株表现出抗菌活性。尽管如此，11 株分离株对革兰氏阳性菌有活性，5 株对革兰氏阴性菌有潜在作用，没有分离株对病原真菌有任何作用。分析了所有 48 个分离株的编码非核糖体肽合成酶 (NRPS) 和聚酮合酶 (PKS) 的基因。在所有 48 个分离株中都检测到 NRPS 序列，28 个分离株中的 PKS-I 和 22 个分离株中的 PKS-II。PKS-I – PKS-II – NRPS 基因在 17 个分离株中得到鉴定。