ABSTRACT

CFP/YFP-paired FRET is routinely used to estimate intracellular Ca²⁺ concentrations in vivo. This system, however, is excited with blue light, which is likely to invoke unexpected responses in plant cells. This report describes a new green light-excitable FRET system with an mKO2/mCherry pair. Plant cells expressing this newly constructed FRET system demonstrated its ability to monitor changes in cytosolic free calcium concentration. The new system is likely to find applications in studies of plant cells where undesirable blue light responses must be avoided.

中文翻译：

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用于监测植物细胞中细胞内钙水平的绿光可激发 FRET 系统

摘要

CFP/YFP 配对 FRET 通常用于估计体内细胞内 Ca ²⁺浓度。然而，该系统受到蓝光的激发，这可能会在植物细胞中引起意想不到的反应。本报告描述了一种带有 mKO2/mCherry 对的新型绿光可激发 FRET 系统。表达这种新构建的 FRET 系统的植物细胞展示了其监测细胞溶质游离钙浓度变化的能力。新系统可能会应用于必须避免不良蓝光反应的植物细胞研究中。