Measuring 3D Cell Culture Viability in Multiple 3D Printed Scaffolds Within a Single Miniature Electrical Impedance Tomography Sensor,Advanced Engineering Materials

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Measuring 3D Cell Culture Viability in Multiple 3D Printed Scaffolds Within a Single Miniature Electrical Impedance Tomography Sensor
Advanced Engineering Materials ( IF 3.4 ) Pub Date : 2021-08-06 , DOI: 10.1002/adem.202100338
Ryoma Ogawa ₁ , Amelia Hallas-Potts ₂ , Hancong Wu ₃ , Jiabin Jia ₄ , Pierre O. Bagnaninchi ₁

Affiliation

The use of 3D cell culture for tissue engineering and regenerative medicine applications often challenges conventional biochemical and optical assays. Impedance-based cellular assays have shown their potential to retrieve dielectric parameters pertaining to cell behavior such as viability, proliferation, and differentiation for 2D adherent cell culture. Herein, simultaneous 3D impedance imaging and viability measurements of multiple large (>2 mm) 3D cell cultures embedded in collagen gels are demonstrated. The method is facilitated by low-resistance 3D printed scaffolds that can hold a 3D cell–gel system throughout cell culture while being transparent to impedance imaging. It is shown in silico and in vitro that frequency-difference electrical impedance tomography (fd-EIT) can nondestructively and in a label-free way differentiate a variety of cell concentrations with a single miniature sensor in real time. This study paves the way toward the development of EIT imaging for the quantitative and noninvasive evaluation of tissue engineering products.

中文翻译：

在单个微型电阻抗断层扫描传感器内测量多个 3D 打印支架的 3D 细胞培养活力

将 3D 细胞培养用于组织工程和再生医学应用通常会挑战传统的生化和光学分析。基于阻抗的细胞检测已显示出它们在检索与细胞行为相关的介电参数（如二维贴壁细胞培养的活力、增殖和分化）方面的潜力。在此，展示了嵌入胶原凝胶中的多个大型（> 2 毫米）3D 细胞培养物的同步 3D 阻抗成像和活力测量。该方法由低电阻 3D 打印支架促进，该支架可以在整个细胞培养过程中保持 3D 细胞凝胶系统，同时对阻抗成像透明。计算机和体外实验表明，频差电阻抗断层扫描 (fd-EIT) 可以使用单个微型传感器以非破坏性和无标记的方式实时区分各种细胞浓度。这项研究为开发用于组织工程产品的定量和无创评估的 EIT 成像铺平了道路。

更新日期：2021-08-06

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