Growing evidence has demonstrated that long non-coding RNAs (lncRNAs) can function as modulators in the development of triple-negative breast cancer (TNBC). However, the function of lncRNA small nucleolar RNA host gene 8 (SNHG8) in TNBC remains unclear. Therefore, our study aimed at investigating the role of SNHG8 in the proliferation and migration of TNBC cells. SNHG8 expression was evaluated using RT-qPCR assay. Cell proliferation and migration were assessed by EdU, colony formation and Transwell assays. The levels of proteins related to EMT process were examined by western blot assay. The interaction among SNHG8, miR-335-5p and pygopus family PHD finger 2 (PYGO2) was detected by RIP assay, RNA pull down assay and luciferase reporter assay. SNHG8 expression was significantly up-regulated in TNBC cells. SNHG8 silencing obviously inhibited TNBC cell proliferation, migration and EMT process. Moreover, SNHG8 acted as a sponge to sequester miR-335-5p in TNBC cells. Besides, PYGO2 was proven as a target gene of miR-335-5p, and SNHG8 promoted TNBC cell proliferation, migration and EMT process through regulating miR-335-5p and PYGO2. Totally, our study indicated that SNHG8 promoted TNBC cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis.

中文翻译：

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长链非编码RNA SNHG8通过调节miR-335-5p/PYGO2轴增强三阴性乳腺癌细胞增殖和迁移

越来越多的证据表明，长链非编码 RNA (lncRNA) 可以作为三阴性乳腺癌 (TNBC) 发展的调节剂。然而，lncRNA 小核仁 RNA 宿主基因 8（SNHG8）在 TNBC 中的功能仍不清楚。因此，我们的研究旨在研究 SNHG8 在 TNBC 细胞增殖和迁移中的作用。使用 RT-qPCR 测定评估 SNHG8 表达。通过 EdU、集落形成和 Transwell 测定评估细胞增殖和迁移。通过蛋白质印迹法检测与 EMT 过程相关的蛋白质水平。通过RIP测定、RNA下拉测定和荧光素酶报告基因测定检测SNHG8、miR-335-5p和pygopu​​s家族PHD指2（PYGO2）之间的相互作用。SNHG8 表达在 TNBC 细胞中显着上调。SNHG8沉默明显抑制TNBC细胞增殖、迁移和EMT过程。此外，SNHG8 作为海绵在 TNBC 细胞中隔离 miR-335-5p。此外，PYGO2被证明是miR-335-5p的靶基因，SNHG8通过调节miR-335-5p和PYGO2促进TNBC细胞增殖、迁移和EMT过程。总之，我们的研究表明 SNHG8 通过调节 miR-335-5p/PYGO2 轴促进 TNBC 细胞增殖和迁移。