The present study was conducted to evaluate the safety profile of Janma Ghunti Honey in Wistar rats on oral administration for 28 consecutive days. Animals were randomized on the basis of body weight into 6 groups. Three groups received test item (TI) at three different dose levels (3 mL/k g, 6 mL/kg and 12 mL/kg body weight). One group served as high dose satellite reversal group (12 mL/kg). One group each served as the control and satellite control group. Animals were observed for clinical signs of toxicity and mortality at least once daily. Animals from satellite groups were observed for further 14 days without treatment to evaluate delayed occurrence or reversibility of any signs/toxicity. At the end of the study, animals were studied through clinical pathology and necropsy examination. No treatment related mortality was observed in any group. Except in female low dose and female high dose satellite groups, all the treated groups exhibited weight gain and no statistically significant alterations were observed. No TI related toxicity was found on hematological investigation, blood biochemistry parameters and absolute and relative organ weights among treated groups. 'No Observed Adverse Effect Level' of TI in male and female Wistar rats was found to be above 12 mL/kg body weight.

中文翻译：

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Janma Ghunti 蜂蜜对 Wistar 大鼠的亚慢性口服毒性研究

本研究旨在评估 Janma Ghunti 蜂蜜在 Wistar 大鼠中连续 28 天口服给药的安全性。根据体重将动物随机分为6组。三组接受三种不同剂量水平（3 mL/kg、6 mL/kg 和 12 mL/kg 体重）的测试项目 (TI)。一组作为高剂量卫星逆转组（12 mL/kg）。一组分别作为对照组和卫星对照组。每天至少一次观察动物的毒性和死亡率的临床症状。来自卫星组的动物在未经治疗的情况下再观察 14 天，以评估任何迹象/毒性的延迟发生或可逆性。在研究结束时，通过临床病理学和尸体剖检对动物进行研究。在任何组中均未观察到与治疗相关的死亡率。除女性低剂量和女性高剂量卫星组外，所有治疗组均表现出体重增加，未观察到统计学上的显着变化。在血液学调查、血液生化参数以及治疗组之间的绝对和相对器官重量方面没有发现与 TI 相关的毒性。发现雄性和雌性 Wistar 大鼠 TI 的“未观察到不良反应水平”高于 12 mL/kg 体重。