The present study deals with oxidative stress in rat kidneys upon administration of Isoniazid (INH) and Rifampicin (RIF), and the attenuative effect of polyherbal formulation Heptoplus. Heptoplus is a polyherbal drug that contains phyllanthin as the active ingredient. Rats were intoxicated with INH and RIF and Heptoplus was orally fed to rats along with INH and RIF. Body weight, serum biochemical, antioxidant status, mitochondrial enzyme activity, lysosomal enzyme leakage and the histopathological impact were studied in the rat kidneys. Body and kidney weight of untreated rats showed deviation, but on treatment, they maintained their normal weight. Untreated rats showed altered serum urea and creatinine levels with deviated antioxidant status and enzymes leakages from lysosomes and decreased mitochondrial enzyme activity in kindey. But Heptoplus treatment allowed the rats to maintain their biochemical indices in blood and kidney at near normal level. Histopathological reports revealed INH- and RIF -induced oxidative stress was not in sufficient amount to incur any significant damage at the tissue level. Hence, both treated and untreated rat kidneys exhibits normal architecture. In conclusion, Heptoplus treatment protects the kidney of rats from the INH- and RIF- induced oxidative stress.

中文翻译：

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多药制剂对异烟肼和利福平诱导的大鼠肾脏氧化应激的评价

本研究涉及异烟肼 (INH) 和利福平 (RIF) 给药后大鼠肾脏的氧化应激，以及多草制剂 Heptoplus 的减弱作用。Heptoplus 是一种以叶黄素为活性成分的多草药药物。大鼠用 INH 和 RIF 中毒，Heptoplus 与 INH 和 RIF 一起口服喂给大鼠。在大鼠肾脏中研究了体重、血清生化、抗氧化状态、线粒体酶活性、溶酶体酶泄漏和组织病理学影响。未治疗大鼠的体重和肾脏重量出现偏差，但在治疗后，它们保持正常体重。未经治疗的大鼠表现出血清尿素和肌酐水平改变，抗氧化状态偏离，酶从溶酶体泄漏，线粒体酶活性降低。但 Heptoplus 治疗使大鼠的血液和肾脏生化指标维持在接近正常水平。组织病理学报告显示 INH 和 RIF 诱导的氧化应激不足以在组织水平上引起任何显着损伤。因此，经过处理和未经处理的大鼠肾脏都表现出正常的结构。总之，Heptoplus 治疗保护大鼠肾脏免受 INH 和 RIF 诱导的氧化应激。