Traditional Chinese medicine detoxification prescription Chaihu-jia-Longgu-Muli decoction (CLMD) relieves depressive symptoms in patients withdrawing from methamphetamine. In this study, we assessed the effects of CLMD on methamphetamine withdrawal in rats. A methamphetamine-intoxicated rat model was established. Rats were randomly divided into the control, model, high-dosage, medium-dosage, and low-dosage groups, receiving high, medium, and low doses of CLMD, respectively. Weekly body weight measurements revealed that rats treated with methamphetamine had the lowest body weight. The conditioned place preference experiment revealed that methamphetamine-intoxicated rats stayed significantly longer in the drug-paired chamber than the control rats. However, after administering high-dosage CLMD, the amount of time the rats spent in the drug-paired chamber was significantly less than that of the model rats. Our open-field test revealed that the model group had lower crossing and rearing scores than the control group. Additionally, rats that received CLMD treatment exhibited higher crossing and rearing scores than the model rats. Striatal DA, 5-HT, and β-EP and serum IL-1α and IL-2 concentrations were estimated. Rats in the model group had lower striatal DA, 5-HT, and β-EP and higher serum IL-1α and IL-2 concentrations than those in the control group. High-dosage CLMD administration significantly changed the concentrations of these molecules, such that they approached normal concentrations. In general, CLMD could prevent the development of methamphetamine-induced withdrawal symptoms in rats by increasing the DA, 5-HT, and β-EP and lowering the IL-1α and IL-2 concentrations.

中文翻译：

---

柴胡加龙骨牡蛎汤对甲基苯丙胺条件性位置偏爱大鼠戒断症状的影响


中药解毒方柴胡加龙骨牡蛎汤（CLMD）可缓解冰毒戒断患者的抑郁症状。在这项研究中，我们评估了 CLMD 对大鼠甲基苯丙胺戒断的影响。建立甲基苯丙胺中毒大鼠模型。将大鼠随机分为对照组、模型组、高剂量组、中剂量组和低剂量组，分别接受高、中、低剂量的CLMD。每周体重测量显示，用甲基苯丙胺治疗的老鼠体重最低。条件性位置偏好实验表明，甲基苯丙胺中毒的大鼠在药物配对室中停留的时间明显长于对照大鼠。然而，给予高剂量CLMD后，大鼠在药物配对室中度过的时间明显少于模型大鼠。我们的旷场测试显示，模型组的杂交和饲养分数低于对照组。此外，接受 CLMD 治疗的大鼠比模型大鼠表现出更高的交叉和饲养分数。估计纹状体 DA、5-HT 和 β-EP 以及血清 IL-1α 和 IL-2 浓度。模型组大鼠纹状体DA、5-HT、β-EP浓度低于对照组，血清IL-1α、IL-2浓度高于对照组。高剂量 CLMD 给药显着改变了这些分子的浓度，使其接近正常浓度。一般来说，CLMD可以通过增加DA、5-HT和β-EP以及降低IL-1α和IL-2浓度来预防大鼠中甲基苯丙胺诱导的戒断症状的发生。