Abstract

In this study, novel single nucleotide polymorphisms (SNPs) were found in the 5′-regulatory regions (promoters) of the bovine glucose transporter (GT) genes SLC2A12 and SLC5A1. These polymorphisms were shown to associate with certain milk production traits in HF cows, including milk yield, milk composition, and somatic cell count. It was shown that the SNP g.-671C > G (NC_037336.1: g.72224078C > G) in the SLC2A12 gene could be an effective marker of cattle production traits and that genotypes CC and CG are associated with the best productivity. The polymorphisms found in the SLC5A1 gene promoter also influenced milk production traits in HF cows, albeit to a lesser extent, and we propose that these polymorphisms could be useful as genetic markers for milk production traits in marker-assisted selection (MAS); however, this must be confirmed on larger populations of cattle. In addition, the presence of polymorphisms within promoter regions appears to affect the expression of GT genes in the cow mammary gland and modify transcription factor (TF) binding capacity.

摘要

在这项研究中，在牛葡萄糖转运蛋白 (GT) 基因SLC2A12和SLC5A1 的 5'-调控区（启动子）中发现了新的单核苷酸多态性 (SNP) 。这些多态性显示与 HF 奶牛的某些产奶特性相关，包括产奶量、奶成分和体细胞计数。结果表明，SLC2A12基因中的 S​​NP g.-671C > G (NC_037336.1: g.72224078C > G) 可能是牛生产性状的有效标记，基因型 CC 和 CG 与最佳生产力相关。在SLC5A1中发现的多态性基因启动子也影响 HF 奶牛的产奶性状，尽管影响程度较小，我们建议这些多态性可用作标记辅助选择 (MAS) 中产奶性状的遗传标记；然而，这必须在更多的牛群中得到证实。此外，启动子区域内多态性的存在似乎会影响奶牛乳腺中 GT 基因的表达并改变转录因子 (TF) 结合能力。