Abstract

An investigation was carried out on Deoni animals of western India to study the allelic and genotypic frequencies in coding region of TYR gene as well as gene expression profile. The animals were grouped according to age, gender, strain and intensity of partial albinism (low, medium and high). The present study revealed that the genotypic frequency of TYR gene across different strains, gender, age group and level of partial albinism was found to be non-significant for both exon-I and exon-II. The AB genotype in Balankya (0.70) was observed highest genotypic frequency followed by Wanera (0.55) and Shewara (0.55) strains. The genotypic frequency of AB and BB genotypes were observed highest in male and female, respectively. In exon-I, genotype frequency of AA genotype was found highest (0.55) in low level of partial albinism. The allelic frequencies in Shewara strain, male and low level of partial albinism were 0.75, 0.63 and 0.73, respectively. However, in exon-II genotype frequency of AB and BB was observed highest (0.70) in Wanera and Balankya strains followed by AA genotype in Shewara (0.50). The highest genotypic frequency of AA (0.87) and BB (0.50) were in male and female, respectively. The genotype frequency of AB genotype was found highest in all level of partial albinism. The allelic frequency was highest (0.85 for B allele) in Wanera strain, male (0.80 for A allele) and high level (0.60 for A allele) of particle albinism. The highly significant (p = 0.002) expression of tyrosinase gene was observed in young animals as compared to adult animals. The TYR gene expression was significantly (p = 0.047) higher in animals with low intensity of partial albinism followed by in the animals with medium and high intensity. Therefore, it is inferred that the TYR gene expression in young animals were high and as compared to the old animals of Deoni cattle breed.

摘要

对印度西部的德奥尼动物进行了一项调查，研究了 TYR 基因编码区的等位基因和基因型频率以及基因表达谱。根据年龄、性别、应变和部分白化病的强度（低、中和高）对动物进行分组。本研究表明，发现 TYR 基因在不同品系、性别、年龄组和部分白化病水平之间的基因型频率对于外显子 I 和外显子 II 均无显着意义。观察到 Balankya (0.70) 中的 AB 基因型基因型频率最高，其次是 Wanera (0.55) 和 Shewara (0.55) 菌株。AB 和 BB 基因型的基因型频率分别在男性和女性中观察到最高。在外显子-I 中，发现 AA 基因型的基因型频率在低水平部分白化病中最高 (0.55)。Shewara品系、雄性和低水平部分白化病的等位基因频率分别为0.75、0.63和0.73。然而，在 Wanera 和 Balankya 菌株中观察到 AB 和 BB 的外显子 II 基因型频率最高 (0.70)，其次是 Shewara 中的 AA 基因型 (0.50)。AA (0.87) 和 BB (0.50) 的最高基因型频率分别在男性和女性中。发现AB基因型的基因型频率在所有级别的部分白化病中最高。等位基因频率在 Wanera 品系中最高（B 等位基因为 0.85），雄性（A 等位基因为 0.80）和颗粒白化病的高水平（A 等位基因为 0.60）。高度显着（70) 在 Wanera 和 Balankya 菌株中，随后是 Shewara 中的 AA 基因型 (0.50)。AA (0.87) 和 BB (0.50) 的最高基因型频率分别在男性和女性中。发现AB基因型的基因型频率在所有级别的部分白化病中最高。等位基因频率在 Wanera 品系中最高（B 等位基因为 0.85），雄性（A 等位基因为 0.80）和颗粒白化病的高水平（A 等位基因为 0.60）。高度显着（70) 在 Wanera 和 Balankya 菌株中，随后是 Shewara 中的 AA 基因型 (0.50)。AA (0.87) 和 BB (0.50) 的最高基因型频率分别在男性和女性中。发现AB基因型的基因型频率在所有级别的部分白化病中最高。等位基因频率在 Wanera 品系中最高（B 等位基因为 0.85），雄性（A 等位基因为 0.80）和颗粒白化病的高水平（A 等位基因为 0.60）。高度显着（p  = 0.002) 与成年动物相比，在幼小动物中观察到酪氨酸酶基因的表达。低强度部分白化病动物的 TYR 基因表达显着 ( p  = 0.047) 较高，其次是中度和高强度动物。因此，推断与德奥尼牛品种的年老动物相比，年轻动物的 TYR 基因表达较高。