Abstract

Campylobacteriosis is among the most frequently reported foodborne zoonoses. A total of 848 samples were screened for Campylobacter spp. and occurrence was found to be 8.7%, 2.3% and 1.65% in broiler cecum samples, chicken meat samples and slaughter house environment swabs, respectively. High level of antimicrobial resistance was found against tetracycline (64.1%), doxycycline (54.4%), ampicillin (46.6%), nalidixic acid (42.7%), kanamycin (35.9%), and ciprofloxacin (33.33%). Resistance to co-amoxiclav (19.4%) and erythromycin (21.4%) was less common. The MAR index of the isolates was in the range of 0.11–0.78. Multi-drug resistance was observed in 54.4% of the isolates, with 53.2% C. jejuni and 55.3% C. coli isolates found resistant against three or more classes of antimicrobials. Presence of mutations in gyrA and 23S rRNA genes was investigated, which revealed that all the fluoroquinolone resistant isolates possessed Thr-86-Ile point mutation, whereas only 68% of erythromycin resistant isolates had A2075G mutation. The tetO gene was present in 91.7% tetracycline resistant isolates and bla_OXA-61 gene was detected in 97.9% of the ampicillin resistant isolates. The occurrence of antimicrobial resistant Campylobacter spp. in broiler chicken samples and slaughter house settings is a public health risk and calls for judicial use of antimicrobials.

摘要

弯曲杆菌病是最常报告的食源性人畜共患病之一。总共对 848 个样品进行了弯曲杆菌属筛选。肉鸡盲肠样本、鸡肉样本和屠宰场环境拭子中的发生率分别为 8.7%、2.3% 和 1.65%。四环素（64.1%）、多西环素（54.4%）、氨苄西林（46.6%）、萘啶酸（42.7%）、卡那霉素（35.9%）和环丙沙星（33.33%）的耐药率较高。对联合阿莫西拉夫 (19.4%) 和红霉素 (21.4%) 的耐药性不太常见。分离株的 MAR 指数在 0.11-0.78 范围内。在 54.4% 的分离株中观察到多重耐药性，其中 53.2% 是空肠弯曲菌，55.3% 是大肠杆菌发现分离株对三类或更多类抗菌药物具有耐药性。对gyrA和 23S rRNA 基因突变的存在进行了调查，结果表明所有氟喹诺酮类耐药菌株都具有 Thr-86-Ile 点突变，而只有 68% 的红霉素耐药菌株具有 A2075G 突变。tetO基因存在于 91.7% 的四环素耐药菌株中，而bla _OXA-61基因在 97.9% 的氨苄青霉素耐药菌株中检测到。抗菌素耐药弯曲杆菌属的发生。在肉鸡样品和屠宰场环境中存在这种情况是一种公共健康风险，需要司法使用抗菌剂。