Herein, we report a facile multiplex enzyme-free electrochemical immunoassay for the detection of foodborne pathogens such as Listeria monocytogenes (Lm) using thionine labeled anti-Lm and Enterobacter cloacae (Ec) using ferrocene labeled anti-Ec as redox probes with Au nanoparticles- (NPs) decorated multiwalled carbon nanotubes (AuNPs-MWCNTs) composites as a detection platform. These nanocomposites have been characterized by Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray analysis (EDAX) techniques. The individual linear range for Lm and Ec detection has been estimated to vary from 10¹–10¹¹ and 10¹–10¹² colony forming units (CFU)/mL, respectively. Conversely, the limit of detection (LOD) for Lm and Ec has been estimated to be 3.98 and 5.39 CFU/mL, respectively. Similarly, the multiplex detection of Lm and Ec showed a linear range of 10¹–10⁷ (Lm) and 10¹–10⁶ CFU/mL (Ec) with the LOD of 3.22 (Lm) and 4.17 CFU/mL (Ec). We have also demonstrated the application of our sandwich electrochemical immunoassay for the detection of Lm in Caenorhabditis elegans and Ec in milk and juice samples.

中文翻译：

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使用金纳米粒子装饰的多壁碳纳米管对食源性病原体进行无酶多重检测

在此，我们报告了一种简便的多重无酶电化学免疫分析，用于使用硫氨酸标记的抗Lm和阴沟肠杆菌( Ec )检测食源性病原体，如单核细胞增生李斯特菌( Lm )，使用二茂铁标记的抗Ec作为氧化还原探针与 Au 纳米粒子- (NPs) 装饰的多壁碳纳米管 (AuNPs-MWCNTs) 复合材料作为检测平台。这些纳米复合材料已通过拉曼光谱、X 射线衍射 (XRD)、扫描电子显微镜 (SEM)、透射电子显微镜 (TEM) 和能量色散 X 射线分析 (EDAX) 技术进行表征。Lm和Ec的单独线性范围估计检测值分别为 10 ¹ –10 ¹¹和 10 ¹ –10 ¹²菌落形成单位 (CFU)/mL。相反，Lm和Ec的检测限 (LOD)估计分别为 3.98 和 5.39 CFU/mL。同样，Lm和Ec的多重检测显示线性范围为 10 ¹ –10 ⁷ ( Lm ) 和 10 ¹ –10 ⁶ CFU/mL ( Ec )，LOD 为 3.22 ( Lm ) 和 4.17 CFU/mL ( Ec )）。我们还展示了我们的三明治电化学免疫的用于检测的应用Lm的在秀丽隐杆线虫和的Ec牛奶和果汁样品英寸