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Effects of a fully enclosed hollow-fiber centrifugal ultrafiltration technique for laboratory biosafety improvement.
Biotechniques ( IF 2.2 ) Pub Date : 2021-08-05 , DOI: 10.2144/btn-2021-0029
Jing Yang 1 , Weigang Wang 1 , Cuixin Qiang 1 , Yanan Niu 1 , Zhirong Li 1 , Mengqiang Zhao 2 , Ye Jiang 2 , Jing Cao 1 , Xiaoran Niu 1 , Xiaoxuan Liu 1 , Jianhong Zhao 1
Affiliation  

Laboratory biosafety has become a core focus in biological analysis, owing to the frequent occurrence of laboratory-acquired infections caused by the leakage of pathogenic microorganisms. For this purpose, the authors developed a safe pretreatment device combining a sealing technique with a direct injection technique. In this study, several bacteria and viruses were used to validate the filtration effect of the invention. Data show that the new device can completely filter bacteria and that the filtration rates for hepatitis B virus and hepatitis C virus reached 94% and 96%, respectively. The results show that the new preparation device can effectively block these pathogens and can improve biological safety and provide powerful protection for technicians.

中文翻译:

全封闭中空纤维离心超滤技术对实验室生物安全改进的影响。

由于病原微生物泄漏引起的实验室获得性感染频繁发生,实验室生物安全已成为生物分析的核心焦点。为此,作者开发了一种结合密封技术和直接注射技术的安全预处理装置。在这项研究中,使用了几种细菌和病毒来验证本发明的过滤效果。数据显示,新装置可以完全过滤细菌,对乙肝病毒和丙肝病毒的过滤率分别达到94%和96%。结果表明,新制备装置能有效阻断这些病原体,提高生物安全性,为技术人员提供有力保障。
更新日期:2021-08-05
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