3D bioprinting technology displays many advantages for tissue engineering applications, but its utilization is limited by veryfew bioinks available for biofabrication. In this study, a novel type of bioink, which includes three methacryloyl modifiedmannans, was introduced to 3D bioprinting for tissue engineering applications. Yeast mannan (YM) was modified by reactingwith methacrylate anhydride (MA) at different concentrations, and three YM derived bioinks were obtained, which weretermed as YM-MA-1, YM-MA-2 and YM-MA-3 and were distinguished with different adjusted methacrylation degrees. TheYM derived bioink displayed an advantage that the mechanical properties of its photo-cured hydrogels can be enhanced withits methacrylation degree. Hence, YM derived bioinks are fitted for the mechanical requirements of most soft tissueengineering, including cartilage tissue engineering. By selecting chondrocytes as the testing cells, well cytocompatibility of YM-MA-1, YM-MA-2 had been confirmed by CCK-8 method. Following photo-crosslinking and implantation into SD rats for 4 weeks, the in vivo biocompatibility of the YM-MA-2 hydrogel is acceptable for tissue engineering applications. Hence, YM-MA-2 was chosen for 3D bioprinting. Our data demonstrated that hydrogel products with designed shape and living chondrocytes have been printed by applying YM-MA-2 as the bioink carrying chondrocytes. After the YM-MA-2 hydrogel with encapsulated chondrocytes was implanted subcutaneously in nude mice for 2 weeks, GAG and COLII secretion was confirmed by histological staining in YM-MA-2-H, indicating that the YM derived bioink can be potentially applied to tissue engineering by employing a 3D printer of stereolithography.

3D 生物打印技术在组织工程应用中显示出许多优势，但其应用受到可用于生物制造的极少生物墨水的限制。在这项研究中，一种新型的生物墨水（包括三种甲基丙烯酰修饰的甘露聚糖）被引入用于组织工程应用的 3D 生物打印。通过与不同浓度的甲基丙烯酸酐（MA）反应对酵母甘露聚糖（YM）进行改性，得到了三种YM衍生的生物墨水，分别称为YM-MA-1、YM-MA-2和YM-MA-3，分别以不同的调节甲基丙烯酸化度。YM衍生的生物墨水显示出一种优势，即其光固化水凝胶的机械性能可以随着其甲基丙烯酸化程度而增强。因此，YM 衍生的生物墨水适合大多数软组织工程的机械要求，包括软骨组织工程。通过选择软骨细胞作为受试细胞，CCK-8法证实YM-MA-1、YM-MA-2具有良好的细胞相容性。在光交联并植入 SD 大鼠 4 周后，YM-MA-2 水凝胶的体内生物相容性可用于组织工程应用。因此，选择 YM-MA-2 进行 3D 生物打印。我们的数据表明，通过应用 YM-MA-2 作为携带软骨细胞的生物墨水，可以打印出具有设计形状和活软骨细胞的水凝胶产品。将包裹有软骨细胞的 YM-MA-2 水凝胶植入裸鼠皮下 2 周后，通过 YM-MA-2-H 中的组织学染色证实 GAG 和 COLII 的分泌，表明 YM 衍生的生物墨水可以潜在地应用于通过使用立体光刻的 3D 打印机进行组织工程。