Sphingomonas sp. Shah is a bacterium that was first isolated from mammalian cell cultures. According to ribotyping data it is very much homologous to the clinically important pathogen Sphingomonas paucimobilis, which has generated pseudo-outbreaks. Using a tissue culture system, Sphingomonas sp. Shah was discovered to induce apoptosis in human lung epithelial carcinoma. Apoptosis of infected cells was determined by numerous criteria including (1) visual alterations in cellular morphology, (2) initiation of nuclear marginalization and chromatin compaction condensation, (3) the attendance of a high percentage of cells with subG1 DNA content, and (4) caspase-3 activation. In the current study we demonstrate the induction of apoptosis in mammalian lung epithelial cells upon infection with Sphingomonas sp. Shah and provide insight into the molecular processes triggering apoptosis.

中文翻译：

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鞘氨醇单胞菌诱导人肺上皮细胞凋亡。Shah，一种最近发现的细胞培养污染物。

鞘氨醇单胞菌属 Shah 是一种最初从哺乳动物细胞培养物中分离出来的细菌。根据核糖分型数据，它与临床上重要的病原体 Sphingomonas paucimobilis 非常同源，后者已产生假爆发。使用组织培养系统，Sphingomonas sp。发现 Shah 在人肺上皮癌中诱导细胞凋亡。受感染细胞的凋亡由许多标准确定，包括 (1) 细胞形态的视觉改变，(2) 核边缘化和染色质压实凝聚的开始，(3) 存在高百分比的具有 subG1 DNA 含量的细胞，以及 (4 ) caspase-3 激活。在目前的研究中，我们证明了在感染鞘氨醇单胞菌后诱导哺乳动物肺上皮细胞凋亡。