In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions.

在 COVID-19 大流行的背景下，EVA-GLOBAL 等病毒库在病毒和相关研究产品的供应中发挥着关键作用。病毒的冷冻干燥技术代表了一种保存菌株及其分布的选择方法，无需苛刻的冷链。在这里，我们描述了一种优化的冻干方案，可用于 SARS-CoV-2 菌株，可改善保存和热稳定性。我们表明，用作佐剂的蔗糖是一种简单有效的稳定剂，可为病毒在不同气候条件下的长期保存和运输提供更多保护。