Abstract

Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique. The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis. The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively. TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex. SEM analysis confirmed the uniform pore distribution of PCGO scaffolds. Moreover, it showed that the pore size of the scaffolds was decreased with the increase in GO content. Among the developed PCGO scaffolds, the scaffolds with 1 wt.% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°. In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation. The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability. These results demonstrate the suitability of PCGO scaffolds for tissue engineering.

摘要

通过冷冻干燥技术制备了基于氧化石墨烯 (GO) 结合果胶/壳聚糖聚电解质复合物 (PCGO) 的三维 (3D) 多孔支架。通过FTIR和XRD分析研究了制备的PCGO支架的化学成分和微观结构。GO 的存在及其在聚合物基质中的均匀分布分别通过拉曼光谱和共焦拉曼映射分析得到证实。TGA 分析表明，GO 的添加提高了果胶/壳聚糖复合物的热稳定性。SEM 分析证实了 PCGO 支架的均匀孔分布。此外，它表明支架的孔径随着 GO 含量的增加而减小。在开发的 PCGO 支架中，1 wt. % 的 GO 通过表现出 2004% 的水溶胀度、1101% 的保水能力和 21° 的水接触角 (WCA) 表现出改善的亲水性。此外，这些支架表现出更好的抗压强度（~283 kPa）和对溶菌酶介导的降解的抵抗力。PCGO 支架表现出可接受水平的生物和血液相容性以及 GO 浓度依赖性细胞附着能力。这些结果证明 PCGO 支架适用于组织工程。PCGO 支架表现出可接受水平的生物和血液相容性以及 GO 浓度依赖性细胞附着能力。这些结果证明 PCGO 支架适用于组织工程。PCGO 支架表现出可接受水平的生物和血液相容性以及 GO 浓度依赖性细胞附着能力。这些结果证明 PCGO 支架适用于组织工程。