Optimal tip sonication settings, namely tip position, input power, and pulse durations, are necessary for temperature sensitive procedures like preparation of viable cell extract. In this paper, the optimum tip immersion depth (20–30% height below the liquid surface) is estimated which ensures maximum mixing thereby enhancing thermal dissipation of local cavitation hotspots. A finite element (FE) heat transfer model is presented, validated experimentally with (R² > 97%) and used to observe the effect of temperature rise on cell extract performance of Escherichia coli BL21 DE3 star strain and estimate the temperature threshold. Relative yields in the top 10% are observed for solution temperatures maintained below 32°C; this reduces below 50% relative yield at temperatures above 47°C. A generalized workflow for direct simulation using the CONSOL code as well as master plots for estimation of sonication parameters (power input and pulse settings) is also presented.

中文翻译：

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使用有限元模型优化大肠杆菌尖端超声处理以获得高产无细胞提取物


对于温度敏感的程序（例如活细胞提取物的制备）来说，最佳的尖端超声设置（即尖端位置、输入功率和脉冲持续时间）是必要的。在本文中，估计了最佳尖端浸入深度（液体表面以下 20-30% 的高度），以确保最大程度的混合，从而增强局部空化热点的散热。提出了有限元（FE）传热模型，并用（ R ² > 97%）进行了实验验证，并用于观察温度升高对大肠杆菌BL21 DE3星株细胞提取性能的影响并估计温度阈值。当溶液温度保持在 32°C 以下时，观察到前 10% 的相对产率；当温度高于 47°C 时，相对产量会降低 50% 以下。还介绍了使用 CONSOL 代码进行直接模拟的通用工作流程以及用于估计超声处理参数（功率输入和脉冲设置）的主图。