Abstract

Context

The roots of Tagetes lucida Cav. (Asteraceae) have antioxidant and antimicrobial properties.

Objective

This study aimed to examine the hepatoprotective effects of T. lucida roots ethanol extract (TLRE) using carbon tetrachloride (CCl₄)-induced hepatotoxicity in rats.

Materials and methods

The active ingredients of TLRE were identified by high-performance liquid chromatography, infra-red spectrum, and mass spectrometric procedures. Ninety rats were distributed into four main groups: positive, therapeutic, protective, and negative group. The therapeutic group was implemented using CCl₄ (a single dose of 2 mL/kg) before TLRE or silymarin administration. Meanwhile, the protective group was implemented by administering CCl₄ (a single dose of 2 mL/kg) after force-feeding TLRE or silymarin. Each therapeutic and protective group was divided into three subgroups: force-fed with saline, TLRE (500 mg/kg), and silymarin (25 mg/kg). The positive group was split into two subgroups that were force-fed TLRE and silymarin. Positive, therapeutic, and protective groups were compared to the negative group (untreated rats). CCl_4, TLRE, and silymarin were orally administrated using a gastric tube.

Results

In the therapeutic and protective groups, TLRE significantly reduced liver enzymes, i.e., aspartate aminotransferase (12.47 and 6.29%), alanine aminotransferase (30.48 and 11.39%), alkaline phosphatase (17.28 and 15.90%), and cytochrome P450-2E1 (39.04 and 48.24%), and tumour necrosis factor-α (53.72 and 53.72%) in comparison with CCl₄-induced hepatotoxicity controls.

Conclusions

TLRE has a potent hepatoprotective effect with a good safety margin. After a repeated study on another type of small experimental animal, their offspring, and an experiment with a large animal, this study may lead to clinical trials.

中文翻译：

---

万寿菊根提取物通过改善氧化应激对四氯化碳诱导的 Wistar 白化大鼠肝毒性的保肝作用

摘要

语境

Tagetes lucida Cav的根。（菊科）具有抗氧化和抗菌特性。

客观的

本研究旨在使用四氯化碳 (CCl ₄ ) 诱导的大鼠肝毒性研究灵芝根乙醇提取物 (TLRE) 的保肝作用。

材料和方法

TLRE 的活性成分通过高效液相色谱、红外光谱和质谱程序进行鉴定。90 只大鼠分为四个主要组：阳性组、治疗组、保护组和阴性组。治疗组在TLRE或水飞蓟素给药前使用CCl ₄（单剂量2 mL / kg）实施。同时，保护组通过给予CCl ₄（单次剂量 2 mL/kg）强喂 TLRE 或水飞蓟素后。每个治疗组和保护组又分为三个亚组：生理盐水灌食、TLRE (500 mg/kg) 和水飞蓟素 (25 mg/kg)。阳性组被分成两个亚组，分别强制喂食 TLRE 和水飞蓟素。将阳性组、治疗组和保护组与阴性组（未治疗的大鼠）进行比较。CCl _4、 TLRE 和水飞蓟素使用胃管口服给药。

结果

在治疗组和保护组中，TLRE 显着降低了肝酶，即天冬氨酸氨基转移酶（12.47 和 6.29%）、丙氨酸氨基转移酶（30.48 和 11.39%）、碱性磷酸酶（17.28 和 15.90%）和细胞色素 P450-2E1（39.04 和 6.29%） 48.24%）和肿瘤坏死因子-α（53.72 和 53.72%）与 CCl ₄诱导的肝毒性对照相比。

结论

TLRE 具有强大的保肝作用，具有良好的安全范围。在对另一种小型实验动物及其后代进行反复研究，并用大型动物进行实验后，这项研究可能会导致临床试验。