Unlike classical HLA class I genes, MR1 is assumed to have limited polymorphic positions. We developed a MR1 specific PCR assay and sequenced 56 DNA samples from cells with a diverse set of HLA genotypes. In this relatively small panel we found six allele groups encoding for different MR1 proteins. The two most frequent allele groups found in this panel had a frequency of 71% (MR1*01) and 25% (MR1*02), respectively. Moreover, the panel contained many intronic SNPs and silent variants, with individual samples containing up to 15 heterozygous positions. The data presented here is consistent with marked variation in MR1.

中文翻译：

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MR1 包含至少六个具有编码区改变的等位基因组

与经典的 HLA I 类基因不同，假定 MR1 具有有限的多态性位置。我们开发了一种 MR1 特异性 PCR 检测方法，并对来自具有不同 HLA 基因型的细胞的 56 个 DNA 样本进行了测序。在这个相对较小的小组中，我们发现了六个编码不同 MR1 蛋白的等位基因组。在该面板中发现的两个最常见的等位基因组的频率分别为 71% ( MR1*01 ) 和 25% ( MR1*02 )。此外，该组包含许多内含子 SNP 和沉默变体，其中单个样本包含多达 15 个杂合位置。这里提供的数据与 MR1 的显着变化一致。