The small GTPase Ran is a member of the Ras superfamily of small GTP-binding proteins, which plays a key role in the translocation of RNA and proteins through the nuclear pore complex. In this study, the full-length cDNA sequence of LmRan gene was obtained, which consists of 648-nucleotides open reading frame (ORF) and encodes 215 amino acids. RT-qPCR results revealed that LmRan was expressed in all developmental days and tissues investigated. Injection of dsLmRan into 4th and 5th instar nymphs, resulted in a significant down-regulation of LmRan transcripts, respectively. All dsLmRan-injected nymphs died before molting. Further hematoxylin and eosin staining of the integument showed that there was no apolysis occurred after silencing LmRan. In addition, the weight of dsLmRan-injected nymphs was significantly lower than that of the control group, and the gastric caecum and midgut was severely smaller. Especiallly, the mRNA level of LmCYP302a1, LmCYP315a1 and LmCYP314a1 responsible for 20E synthesis, LmE75 and LmE74 genes involved in the 20E signaling pathway, LmGfat, LmUAP1 and LmCHT10 genes involved in chitin metabolism pathway were dramatically decreased in the dsLmRan-injected nymphs. Together, the results indicated that LmRan participate in the 20E signaling pathway, which is essential for the growth and development of locusts.

小 GTPase Ran 是小 GTP 结合蛋白 Ras 超家族的成员，在 RNA 和蛋白质通过核孔复合物的易位中起关键作用。本研究获得了LmRan基因的全长cDNA序列，由648个核苷酸的开放阅读框（ORF）组成，编码215个氨基酸。RT-qPCR 结果显示LmRan在所有发育日和研究的组织中均有表达。将 ds LmRan注射到 4 龄和 5 龄若虫中，分别导致LmRan转录本的显着下调。所有 ds LmRan- 被注射的若虫在蜕皮前死亡。对外皮的进一步苏木精和伊红染色表明在使LmRan沉默后没有发生分离。此外，注射ds LmRan的若虫体重明显低于对照组，胃盲肠和中肠明显变小。Especiallly，的mRNA水平LmCYP302a1，LmCYP315a1和LmCYP314a1负责20E合成，LmE75和LmE74参与20E信号传导途径的基因，LmGfat，LmUAP1和LmCHT10参与几丁质代谢途径基因被显着地在DS降低LmRan-注入若虫。总之，结果表明LmRan参与了 20E 信号通路，这对蝗虫的生长和发育至关重要。