Ovarian cancer causes more deaths than any other cancer of the female reproductive system because there is no effective screening and most women are diagnosed at advanced stages. The probability of survival at 5 years is less than 30%, and the limitation is that it will not respond to chemotherapy protocol and surgery as well. Moreover, some evidence have shown potential anticancer properties of flavonoids, protective chemicals in plant foods, such as being an antioxidant, antiestrogenic, antiproliferative, and antiinflammatory. In this study, the anticancer activity of crude ethanol extracts of leaves from Adhatoda vasica was investigated. By the application of a cell-based assay, the LC 50 value of the A. vasica which showed anticancer effect was used for further studies. The cell line treated with LD 50 value of A. vasica extracts was observed for 0 h, 24 h, and 48 h to reveal the inhibition of the metastatic property in treated PA1 cells. The mRNA isolated from the teratocarcinoma PA1 cells treated with the A. vasica extract was further converted to cDNA and was amplified for the analysis of the p53 gene, p21 gene, and GAPDH gene expression. The expression in treated cells and the untreated control indicated the activity of the A. vasica extract against the ovarian cancer. The present study suggested the antiproliferative and antimetastatic effects of medicinal plant A. vasica on PA1 cells.

中文翻译：

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虎杖乙醇提取物对人卵巢癌细胞系的体外抗癌活性

卵巢癌导致的死亡人数比任何其他女性生殖系统癌症都要多，因为没有有效的筛查方法，而且大多数女性在确诊时已处于晚期。5 年生存概率小于 30%，限制是它对化疗方案和手术也没有反应。此外，一些证据表明类黄酮具有潜在的抗癌特性，植物性食物中的保护性化学物质，如抗氧化剂、抗雌激素、抗增殖和抗炎。在这项研究中，研究了 Adhatoda vasica 叶子的粗乙醇提取物的抗癌活性。通过应用基于细胞的测定，将显示抗癌作用的A. vasica 的LC 50 值用于进一步研究。用 A 的 LD 50 值处理的细胞系。观察 vasica 提取物 0 小时、24 小时和 48 小时，以揭示对处理的 PA1 细胞转移特性的抑制。从用A. vasica提取物处理的畸胎癌PA1细胞中分离的mRNA被进一步转化为cDNA并被扩增用于p53基因、p21基因和GAPDH基因表达的分析。在处理过的细胞和未处理过的对照中的表达表明了A.vasica提取物对卵巢癌的活性。本研究表明药用植物 A. vasica 对 PA1 细胞具有抗增殖和抗转移作用。p21 基因和 GAPDH 基因表达。在处理过的细胞和未处理过的对照中的表达表明了A.vasica提取物对卵巢癌的活性。本研究表明药用植物 A. vasica 对 PA1 细胞具有抗增殖和抗转移作用。p21 基因和 GAPDH 基因表达。在处理过的细胞和未处理过的对照中的表达表明了A.vasica提取物对卵巢癌的活性。本研究表明药用植物 A. vasica 对 PA1 细胞具有抗增殖和抗转移作用。