Microorganism was very sensitive to external temperature change, which also affected its normal metabolism and secretion. Low temperature exopolysaccharides (LT-EPS) and normal temperature exopolysaccharides (NT-EPS) secreted by Bacillus sp. ZT-1 mediated by temperature were studied in this paper. The total carbohydrate in the LT-EPS and NT-EPS were found to be 82.54 ± 2.56 % and 94.23 ± 1.59 % (w/w). The High Performance Liquid Chromatography (HPLC) revealed the mannose and galacturonic acid accounted for 45.52 and 23.49 % in LT-EPS, respectively. In like manner, mannose and galacturonic acid contained 43.99 and 25.24 % in NT-EPS.

One-dimensional nuclear magnetic resonance (NMR) revealed the connection mode of sugar chains. NT-EPS exhibited higher viscosity, better emulsification properties and the larger molecular weight than LT-EPS. Scanning electron microscopy (SEM) showed that LT-EPS was sheet-like with sugar chain branches while NT-EPS was showed as network structure. Furthermore, the 2812 differentially expressed genes (DEGs) were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and located 739 biological pathways. Finally, transcriptome analysis revealed differences in gene expression of the pentose phosphate pathway of carbohydrate metabolism might be the main reason for this difference.

微生物对外界温度变化非常敏感，这也影响了其正常的代谢和分泌。芽孢杆菌分泌的低温胞外多糖 (LT-EPS) 和常温胞外多糖 (NT-EPS) 。本文研究了温度介导的ZT-1。发现 LT-EPS 和 NT-EPS 中的总碳水化合物为 82.54 ± 2.56 % 和 94.23 ± 1.59 % (w/w)。高效液相色谱 (HPLC) 显示甘露糖和半乳糖醛酸分别占 LT-EPS 的 45.52% 和 23.49%。同样，甘露糖和半乳糖醛酸在 NT-EPS 中的含量分别为 43.99% 和 25.24%。

一维核磁共振（NMR）揭示了糖链的连接方式。NT-EPS 表现出比 LT-EPS 更高的粘度、更好的乳化性能和更大的分子量。扫描电子显微镜（SEM）显示LT-EPS呈片状，带有糖链分支，而NT-EPS呈网络结构。此外，通过京都基因和基因组百科全书（KEGG）富集分析了2812个差异表达基因（DEG），并定位了739条生物通路。最后，转录组分析显示碳水化合物代谢的戊糖磷酸途径基因表达的差异可能是造成这种差异的主要原因。