Skeletal muscle is one of the most important economic traits in the poultry industry whose development goes through several processes influenced by several candidate genes. This study explored the regulatory role of DCN on MSTN and the influence of these genes on the proliferation and differentiation of embryonic myoblasts in Leizhou black ducks. Embryonic myoblasts were transfected with over-expressing DCN, Si-DCN, and empty vector and cultured for 24 h, 48 h, and 72 h of proliferation and the comparative expression of DCN and MSTN were measured. The results showed that cells transfected with the over-expression DCN had a significantly (P < 0.05) higher expression of DCN mRNA than the normal group and the expression of MSTN mRNA showed a downward trend during the proliferation of myoblasts. DCN mRNA expression was lower in cells transfected with Si-DCN than the normal group in all stages of proliferation. While the expression of MSTN in the Si-DCN transfected group was higher than the normal group with a significant (P < 0.05) difference at the 72 h stage. DCN mRNA increased at the early stage of differentiation but decreased (P > 0.05) from the 6th day to the 8th day of differentiation. The level of MSTN increased gradually during the differentiation process of myoblasts until it decreased significantly on the 8th day. These results show that DCN enhances the proliferation and differentiation of Leizhou black duck myoblasts and suppresses MSTN activity.

骨骼肌是家禽业中最重要的经济特征之一，其发展经历了受几个候选基因影响的几个过程。本研究探讨了DCN对MSTN的调控作用以及这些基因对雷州黑鸭胚胎成肌细胞增殖分化的影响。胚胎成肌细胞用过表达的 DCN、Si-DCN 和空载体转染并培养 24 小时、48 小时和 72 小时的增殖，并测量 DCN 和 MSTN 的比较表达。结果表明，转染过表达 DCN 的细胞具有显着（P < 0.05）DCN mRNA表达高于正常组，MSTN mRNA表达在成肌细胞增殖过程中呈下降趋势。在所有增殖阶段，Si-DCN转染细胞的DCN mRNA表达均低于正常组。而Si-DCN转染组MSTN的表达高于正常组， 在72 h阶段差异显着（P < 0.05）。DCN mRNA在分化早期增加但减少（P > 0.05) 从分化的第 6 天到第 8 天。MSTN水平在成肌细胞分化过程中逐渐升高，直至第8天显着下降。这些结果表明DCN增强了雷州黑鸭成肌细胞的增殖和分化并抑制了MSTN活性。